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PMID: 6322131 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Detection of multiple, unspliced precursor mRNA transcripts for the Mr 32,000 thylakoid membrane protein from Euglena gracilis chloroplasts.

Nucleic acids research ·Vol. 12 ·No. 4 ·1984-02-24 ·Pages 2001-17

Hollingsworth MJ, Johanningmeier U, Karabin GD, Stiegler GL, Hallick RB

Abstract

The psbA gene is the coding locus for a polypeptide of 32 kilodaltons that is involved in electron transport through photosystem II. The 4.9 kilobasepair (kbp) EcoRI restriction endonuclease fragment EcoI from the 145 kbp Euglena gracilis chloroplast DNA was shown to encode psbA. Five transcripts of size 3.1, 2.8, 2.3, 1.8, and 1.2 kilobases were detected by hybridization of psbA probes to nitrocellulose filter blots of electrophoretically separated RNAs. This same pattern was observed when the hybridization probe consisted of only exon sequences from this split gene. A synthetic, intron specific probe hybridized to all RNA precursors except the 1.2 kb mature RNA. These results and psbA DNA sequence data lead to the conclusion that the four higher molecular weight transcripts are unprocessed precursors of the 1.2 kilobase RNA, some of which contain unspliced intervening sequences. There is an increase in psbA transcripts during light induced maturation of the chloroplasts.

MeSH Terms
Base Sequence Chloroplasts/metabolism DNA Restriction Enzymes Euglena gracilis/genetics,metabolism Genes Intracellular Membranes/metabolism Membrane Proteins/genetics,isolation & purification Molecular Weight Nucleic Acid Hybridization RNA, Messenger/genetics Transcription, Genetic
Chemicals
Membrane Proteins RNA, Messenger thylakoid polypeptides DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hollingsworth M J
Johanningmeier U
Karabin G D
Stiegler G L
Hallick R B
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.4.2001
Published
1984-02-24
Pages
2001-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318636
Subset
IM
Grants
NIGMS NIH HHS · GM21351 · United States
External Links
PubMed source DOI: 10.1093/nar/12.4.2001 PMC Full Text View journal details
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