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PMID: 6319759 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence that Escherichia coli virus T1 induces a DNA methyltransferase.

Journal of virology ·Vol. 49 ·No. 2 ·1984-02-00 ·Pages 588-90

Auer B, Schweiger M

Abstract

DNA of Escherichia coli virus T1 is resistant to MboI cleavage and appears to be heavily methylated. Analysis of methylation by the isoschizomeric restriction enzymes Sau3AI and DpnI revealed that recognition sites for E. coli DNA adenine methylase (dam methylase) are methylated. The same methylation pattern was found for virus T1 DNA grown on an E. coli dam host, indicating a T1-specific DNA methyltransferase.

MeSH Terms

Coliphages/enzymology,genetics DNA (Cytosine-5-)-Methyltransferases/genetics DNA Restriction Enzymes Enzyme Induction Escherichia coli/enzymology,genetics Genes Genes, Bacterial Genes, Viral Methyltransferases/genetics

Chemicals

Methyltransferases DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Auer B

Schweiger M

References (17)

17 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.49.2.588-590.1984

Published

1984-02-00

Pages

588-90

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC255502

Subset

External Links

PubMed source DOI: 10.1128/JVI.49.2.588-590.1984 PMC Full Text View journal details

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Evidence that Escherichia coli virus T1 induces a DNA methyltransferase.

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