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PMID: 6317895 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mouse mammary tumor virus: specific methylation patterns of proviral DNA in normal mouse tissues.

Journal of virology ·Vol. 49 ·No. 1 ·1984-01-00 ·Pages 66-71

Hu WS, Fanning TG, Cardiff RD

Abstract

The methylation state of endogenous mouse mammary tumor virus (MuMTV) proviral DNA was examined in normal mouse tissues. DNAs from various tissues were cleaved with the methylation-sensitive enzymes HhaI and HpaII and analyzed by Southern blotting. Tissue-specific MuMTV proviral DNA methylation patterns were found in the BALB/c, C3H, C57BL, GR/A, and GR-Mtv-2- mouse strains. MuMTV proviral DNA was hypomethylated in DNAs from the spleens and testes of all strains examined. The GR/A mouse strain, which was most thoroughly studied, also showed hypomethylation of MuMTV proviral DNA in bone marrow and placental tissues. Analysis of RNAs extracted from GR/A liver, mammary tumor, testes, placenta, and spleen tissues demonstrated that MuMTV proviral hypomethylation need not reflect significant proviral transcription.

MeSH Terms
Cell Transformation, Viral Cells, Cultured DNA, Viral/genetics,metabolism Gene Expression Regulation Genes, Viral Mammary Tumor Virus, Mouse/genetics Methylation RNA, Viral/genetics Spleen/microbiology Substrate Specificity Transcription, Genetic
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hu W S
Fanning T G
Cardiff R D
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33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.49.1.66-71.1984
Published
1984-01-00
Pages
66-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255425
Subset
IM
Grants
NCI NIH HHS · 5R01-CA21454 · United States
External Links
PubMed source DOI: 10.1128/JVI.49.1.66-71.1984 PMC Full Text View journal details
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