Home LiteratureArticle Details
PMID: 6317893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and partial chemical characterization of a 64,000-dalton glycoprotein of human cytomegalovirus.

Journal of virology ·Vol. 49 ·No. 1 ·1984-01-00 ·Pages 279-82

Clark BR, Zaia JA, Balce-Directo L, Ting YP

Abstract

A guanidinium chloride extract of [3H]glucosamine- and [35S]methionine-labeled virions plus dense bodies of human cytomegalovirus (Towne) was separated by reverse-phase high-pressure liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the eluate revealed the major peak to be a glycoprotein with a relative mass of 64,000. This glycoprotein (HCMVgp64) was characterized by amino acid analysis and a high-pressure liquid chromatographic map of its tryptic peptides.

MeSH Terms
Amino Acids/analysis Chromatography, High Pressure Liquid Cytomegalovirus/analysis Glycoproteins/isolation & purification Molecular Weight Viral Proteins/isolation & purification
Chemicals
Amino Acids Glycoproteins Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clark B R
Zaia J A
Balce-Directo L
Ting Y P
References (10)
10 references, click to expand
  1. Viscosity-density gradient for purification of foot-and-mouth disease virus.
    Arch Gesamte Virusforsch. 1972;36(1):141-6 PMID: 4335020
  2. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  3. The morphogenesis of human cytomegalovirus. Isolation and polypeptide characterization of cytomegalovirions and dense bodies.
    Virology. 1975 Aug;66(2):464-73 PMID: 168685
  4. Cytomegalovirus proteins. I. Polypeptides of virions and dense bodies.
    J Virol. 1976 Jul;19(1):243-54 PMID: 181595
  5. Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera.
    Infect Immun. 1982 Jun;36(3):933-42 PMID: 6284646
  6. Analysis of structural polypeptides of purified human cytomegalovirus.
    J Virol. 1976 Dec;20(3):604-11 PMID: 186638
  7. Antibody to varicella-zoster virus-induced membrane antigen: immunofluorescence assay using monodisperse glutaraldehyde-fixed target cells.
    J Infect Dis. 1977 Oct;136(4):519-30 PMID: 198487
  8. Two-column system for determination of glucosamine, galactosamine, and amino acids on a Beckman 121MB amino acid analyzer: separation of the anomers of glucosamine and galactosamine.
    Anal Biochem. 1979 Jul 1;96(1):77-83 PMID: 40457
  9. Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants.
    Infect Immun. 1982 Jun;36(3):924-32 PMID: 6178693
  10. Human cytomegalovirus: glycoproteins associated with virions and dense bodies.
    J Virol. 1976 Aug;19(2):594-609 PMID: 183016
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.49.1.279-282.1984
Published
1984-01-00
Pages
279-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255455
Subset
IM
Grants
NCI NIH HHS · CA 30206 · United States
NCI NIH HHS · CA 33572 · United States
External Links
PubMed source DOI: 10.1128/JVI.49.1.279-282.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2