Abstract
Salmonella DNA was partially digested with EcoRI, and the digest was fractionated to obtain fragments larger than 8 kilobases (kb). These were ligated into EcoRI-cut pBR322, and the mixture was used to transform Salmonella Xyl- cells selecting for ampR xyl+ transformants. A 21- and a 27-kb plasmid were isolated, both of which contained the entire xylose regulon. The xylose regulon was localized to a 6.3-kb segment of a 13.5-kb EcoRI fragment. Subclones were constructed which contained either the genes for D-xylose isomerase and D-xylulokinase or the genes for the D-xylose transport and the D-xylose regulatory factors. The gene order determined by the subcloning experiments is consistent with that determined by genetic mapping. The spots corresponding to D-xylose isomerase and D-xylulokinase subunits were identified in two-dimensional gels of several xylose-induced strains. Each of them had a molecular weight of 45,000 and an isoelectric point of 6.2 +/- 0.1.
MeSH Terms
Aldose-Ketose Isomerases
Carbohydrate Epimerases/genetics
Chromosome Mapping
Cloning, Molecular
DNA, Bacterial/genetics
Genes, Bacterial
Genes, Regulator
Phosphotransferases/genetics
Phosphotransferases (Alcohol Group Acceptor)
Plasmids
Salmonella typhimurium/genetics,isolation & purification,metabolism
Transformation, Bacterial
Xylose/genetics,metabolism
Chemicals
DNA, Bacterial
Xylose
Phosphotransferases
Phosphotransferases (Alcohol Group Acceptor)
xylulokinase
Carbohydrate Epimerases
Aldose-Ketose Isomerases
xylose isomerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ghangas G S
Wilson D B
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11 references, click to expand
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