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PMID: 6316348 Published · ppublish English Journal Article

beta-Adrenergic receptor regulation of N-linked protein glycosylation in rat parotid acinar cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 23 ·1983-12-00 ·Pages 7146-50

Kousvelari EE, Grant SR, Baum BJ

Abstract

We have investigated the relationship between beta-adrenergic receptor stimulation and protein glycosylation and secretion in rat parotid gland cells in vitro. The potent beta-adrenergic agonist (-)-isoproterenol increases [3H]mannose incorporation into newly synthesized glycoproteins. This effect is enhanced if cells are first preincubated with dolichyl phosphate and is not observed after muscarinic-cholinergic or alpha-adrenergic stimulation of cells. The increase in [3H]mannose incorporation is abolished by incubation of cells with tunicamycin, suggesting that the glycosylation events being studied involved asparagine-linked oligosaccharides. The extent of increase in glycosylation is dependent on the concentration of (-)-isoproterenol to which cells are exposed. (+/-)-Propanolol totally abolishes the (-)-isoproterenol-induced increase in [3H]mannose incorporation, in a manner similar to its effects on exocrine secretion. Our findings suggest that beta-adrenergic receptor activation has a profound influence on N-linked protein glycosylation in rat parotid cells in addition to eliciting exocrine protein release.

MeSH Terms
Animals Carbachol/pharmacology Carbon Radioisotopes Dolichol Phosphates/pharmacology Epinephrine/pharmacology Glycoproteins/genetics Isoproterenol/pharmacology Leucine/metabolism Male Mannose/metabolism Parotid Gland/metabolism Protein Processing, Post-Translational Rats Rats, Inbred Strains Receptors, Adrenergic, beta/drug effects,metabolism Tritium
Chemicals
Carbon Radioisotopes Dolichol Phosphates Glycoproteins Receptors, Adrenergic, beta Tritium dolichol monophosphate Carbachol Leucine Isoproterenol Mannose Epinephrine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kousvelari E E
Grant S R
Baum B J
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.23.7146
Published
1983-12-00
Pages
7146-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390010
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.23.7146 PMC Full Text View journal details
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