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PMID: 6316256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.

Nucleic acids research ·Vol. 11 ·No. 21 ·1983-11-11 ·Pages 7303-16

Yaoita Y, Matsunami N, Choi CY, Sugiyama H, Kishimoto T, Honjo T

Abstract

We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of the JH-containing fragments have revealed the rearrangement from the preformed D-JH complex to the complete VH-D-JH gene, which was accompanied by the expression of the intra-cytoplasmic mu chain. In one case a JH segment downstream to the preformed D-JH was used to create a new VH-D-JH gene. Upon the D-JH and VH-D-JH rearrangements the intervening D segments were deleted from the chromosome. One of the expressed VH genes suffered from a large deletion of the 3' portion (including the 95th cysteine residue) of the VH segment. We discuss the possible mechanism of the allelic exclusion.

MeSH Terms
Abelson murine leukemia virus/genetics Alleles Amino Acid Sequence Animals Base Sequence Cell Transformation, Neoplastic Cloning, Molecular DNA Restriction Enzymes Genes Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Mice
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Variable Region DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yaoita Y
Matsunami N
Choi C Y
Sugiyama H
Kishimoto T
Honjo T
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.21.7303
Published
1983-11-11
Pages
7303-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326484
Subset
IM
Databases
GENBANK
X01040, X01041, X01042
External Links
PubMed source DOI: 10.1093/nar/11.21.7303 PMC Full Text View journal details
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