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PMID: 6315767 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evaluation of solubilized herpes simplex virus membrane antigen by enzyme-linked immunosorbent assay.

Journal of clinical microbiology ·Vol. 18 ·No. 5 ·1983-11-00 ·Pages 1160-6

Jeansson S, Forsgren M, Svennerholm B

Abstract

An antigen prepared by solubilization of membranes from herpes simplex virus (HSV)-infected cells with deoxycholate was evaluated by enzyme-linked immunosorbent assay. The deoxycholate-solubilized antigen, previously shown to contain all major HSV glycoproteins, was noninfectious and adsorbed easily and reproducibly to a polystyrene surface at pH 9.6. The deoxycholate-solubilized antigen provided an enzyme-linked immunosorbent assay of high sensitivity and reproducibility with complete correlation with complement fixation for the diagnosis of acute HSV infection. The correlation with neutralization and immunofluorescence for the presence or absence of anti-HSV activity was very good. Comparison with an HSV envelope preparation yielded results slightly in favor of the deoxycholate-solubilized antigen. The assay seems to be useful for demonstration of intrathecal production of antibody activity in HSV encephalitis.

MeSH Terms
Animals Antigens, Viral/analysis Cell Line Cell Membrane/immunology Cricetinae Enzyme-Linked Immunosorbent Assay Humans Simplexvirus/immunology Solubility
Chemicals
Antigens, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jeansson S
Forsgren M
Svennerholm B
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17 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.18.5.1160-1166.1983
Published
1983-11-00
Pages
1160-6
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC272860
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.18.5.1160-1166.1983 PMC Full Text View journal details
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