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PMID: 6311876 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens.

Journal of clinical microbiology ·Vol. 18 ·No. 2 ·1983-08-00 ·Pages 445-8

Schmidt NJ, Dennis J, Devlin V, Gallo D, Mills J

Abstract

Direct immunofluorescence and direct immunoperoxidase staining were equally sensitive and specific for detection of herpes simplex virus antigen in lesion specimens, and each method showed 82% agreement with virus isolation results.

MeSH Terms

Antigens, Viral/analysis Female Fluorescent Antibody Technique Herpes Simplex/microbiology Humans Immunoenzyme Techniques Male Simplexvirus/immunology,isolation & purification

Chemicals

Antigens, Viral

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Schmidt N J

Dennis J

Devlin V

Gallo D

Mills J

References (11)

11 references, click to expand

Immunofluorescent staining in the laboratory diagnosis of varicella-zoster virus infections.
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Evaluation of polyvinyl alcohols as semipermanent mountants for fluorescent-antibody studies.
J Bacteriol. 1967 Feb;93(2):768-9 PMID: 4164053
Direct immunofluorescence test for the diagnosis of genital herpesvirus infections.
Appl Microbiol. 1973 Sep;26(3):373-5 PMID: 4356460
Rapid diagnosis and typind of herpesvirus hominis by the fluorescent antibody method.
Scand J Infect Dis. 1974;6(2):113-6 PMID: 4369110
Immunochemical studies of the endocrine cells of the gastrointestinal tract. I. The use and value of peroxidase-conjugated antibody techniques for the localization of gastrin-containing cells in the human pyloric antrum.
Histochem J. 1975 Jul;7(4):321-33 PMID: 807542
A new specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase.
Histochem J. 1977 Nov;9(6):789-92 PMID: 72746
Use of immunoperoxidase for rapid diagnosis of mucocutaneous herpes simplex virus infection.
J Clin Microbiol. 1977 Dec;6(6):571-3 PMID: 201662
Sensitivity and specificity of diagnostic tests for genital infection with herpesvirus hominis.
Sex Transm Dis. 1979 Jan-Mar;6(1):10-3 PMID: 221993
Detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the Papanicolaou stain.
J Clin Microbiol. 1979 Nov;10(5):737-46 PMID: 94600
A rapid, sensitive myeloperoxidase stain using 4-chloro-1-naphthol.
Am J Clin Pathol. 1980 Jun;73(6):797-9 PMID: 6156595
Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection.
J Clin Microbiol. 1981 May;13(5):913-8 PMID: 6263945

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.18.2.445-448.1983

Published

1983-08-00

Pages

445-8

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC270826

Subset

Grants

NIAID NIH HHS · AI-15990 · United States

External Links

PubMed source DOI: 10.1128/jcm.18.2.445-448.1983 PMC Full Text View journal details

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Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens.

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