Isolation and characterisation of DraI, a type II restriction endonuclease recognising a sequence containing only A:T basepairs, and inhibition of its activity by uv irradiation of substrate DNA.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5467-74

Purvis IJ, Moseley BE

Abstract

A type II restriction endonuclease, DraI, isolated from Deinococcus radiophilus ATCC 27603 recognises the palindromic hexanucleotide sequence (formula; see text) and cleaves it, as indicated by the arrows, to produce blunt-ended fragments. The yield of enzyme is 100 to 1000 times that of the only other known type II restriction endonuclease that recognises a sequence composed solely of A:T basepairs, the isoschizomer AhaIII (1). Ultraviolet irradiation of the DNA substrate at relatively low doses inhibits the activity of DraI by "protecting" the recognition sequence and this may be exploited to give control of partial digestion of DNA by DraI.

MeSH Terms

Adenine Bacteria/enzymology Base Composition Base Sequence DNA/radiation effects DNA Restriction Enzymes/isolation & purification,metabolism Deoxyribonucleases, Type II Site-Specific Substrate Specificity Thymine Ultraviolet Rays

Chemicals

DNA DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific TTTAAA -specific type II deoxyribonucleases Adenine Thymine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Purvis I J

Moseley B E

References (15)

15 references, click to expand

Studies on sequence recognition by type II restriction and modification enzymes.
CRC Crit Rev Biochem. 1982;13(3):287-323 PMID: 6293768
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
Gene. 1979 May;6(1):23-8 PMID: 383576
Assaying of organisms for the presence of restriction endonucleases.
Methods Enzymol. 1980;65(1):19-23 PMID: 6246341
A general method for defining restriction enzyme cleavage and recognition sites.
Methods Enzymol. 1980;65(1):391-404 PMID: 6246360
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
A sequence specific endonuclease from Micrococcus radiodurans.
Biochim Biophys Acta. 1982 May 31;697(2):178-84 PMID: 6285977
Restriction enzyme cleavage of ultraviolet-damaged DNA.
Biochim Biophys Acta. 1982 May 31;697(2):255-8 PMID: 6285980
AhaIII: a restriction endonuclease with a recognition sequence containing only A:T basepairs.
FEBS Lett. 1982 Jul 5;143(2):296-300 PMID: 6288466
Blockage of restriction endonuclease cleavage by thymine dimers.
Photochem Photobiol. 1982 Oct;36(4):429-32 PMID: 6292966
Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642-6 PMID: 333444
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Complete nucleotide sequence of SV40 DNA.
Nature. 1978 May 11;273(5658):113-20 PMID: 205802
The nucleotide sequence of bacteriophage phiX174.
J Mol Biol. 1978 Oct 25;125(2):225-46 PMID: 731693
Nucleotide sequence of bacteriophage lambda DNA.
J Mol Biol. 1982 Dec 25;162(4):729-73 PMID: 6221115

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.16.5467

Published

1983-08-25

Pages

5467-74

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326291

Subset

External Links

PubMed source DOI: 10.1093/nar/11.16.5467 PMC Full Text View journal details

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Isolation and characterisation of DraI, a type II restriction endonuclease recognising a sequence containing only A:T basepairs, and inhibition of its activity by uv irradiation of substrate DNA.

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