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PMID: 6307275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The properties of potato (Solanum tuberosum) lectin after deglycosylation by trifluoromethanesulphonic acid.

The Biochemical journal ·Vol. 211 ·No. 1 ·1983-04-01 ·Pages 273-6

Desai NN, Allen AK, Neuberger A

Abstract

Potato (Solanum tuberosum) lectin, which is a very highly glycosylated glycoprotein, has been completely deglycosylated by use of the trifluoromethanesulphonic acid reagent described by Edge, Faltnek, Hof, Reichert & Weber [(1981) Analyt. Biochem. 118, 131-137]. This shows that both hydroxyproline-arabinofuranoside and serine-galactopyranoside linkages are hydrolysed. The deglycosylated lectin is still functional and cross-reacts with one component of an anti-(potato lectin) antiserum.

MeSH Terms
Amino Acids/analysis Carbohydrates/analysis Chemical Phenomena Chemistry Electrophoresis, Polyacrylamide Gel Hemagglutination Tests Immunodiffusion Lectins Mesylates Optical Rotatory Dispersion
Chemicals
Amino Acids Carbohydrates Lectins Mesylates trifluoromethanesulfonic acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Desai N N
Allen A K
Neuberger A
References (11)
11 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2110273
Published
1983-04-01
Pages
273-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1154352
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2110273 PMC Full Text View journal details
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