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PMID: 6306571 Published · ppublish English Journal Article

Immunoglobulin diversity: analysis of the germ-line VH gene repertoire of the murine anti-GAT response.

Nucleic acids research ·Vol. 11 ·No. 12 ·1983-06-25 ·Pages 4007-17

Schiff C, Milili M, Fougereau M

Abstract

A cDNA clone was constructed from a mRNA encoding an anti-GAT (Glu60 Ala30 Tyr10) BALB/c monoclonal antibody heavy chain. Its sequence, covering codons -5 to 162 and therefore encompassing the complete V-D-J region, was determined. Surprisingly, the sequence of the VH gene-encoded region was almost identical with that of the BALB/c VH anti-HNP (4-hydroxy-3-nitrophenyl) acetyl VH region, suggesting that the same VH germ-line might be used to encode two heavy chains contributing to antibodies of discrete specificities. A specific VH probe was derived and annealed to Eco RI and Bg1 II restriction fragments of liver (unrearranged) DNA extracted from the BALB/c, DBA/2 and C57BL/6 mouse strains that differ in their H chain allotypes. Under stringent conditions, only a few bands were identified by Southern blotting. The different patterns observed suggest that the VH anti-GAT repertoire differs between these strains even though their various anti-GAT antibodies express the same public idiotypic specificities.

MeSH Terms
Amino Acid Sequence Animals Antibodies/genetics Antibodies, Monoclonal/genetics Base Sequence DNA/analysis DNA Restriction Enzymes/metabolism Mice Mice, Inbred BALB C Peptides/immunology Polymers
Chemicals
Antibodies Antibodies, Monoclonal Peptides Polymers GAT DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schiff C
Milili M
Fougereau M
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.12.4007
Published
1983-06-25
Pages
4007-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326021
Subset
IM
Databases
GENBANK
K00687
External Links
PubMed source DOI: 10.1093/nar/11.12.4007 PMC Full Text View journal details
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