Human T-cell hybridomas producing migration inhibitory factor and macrophage activating factors.

Studies were carried out to determine the heterogeneity of factors that affect macrophage functions using human hybridomas constructed by fusing PHA-activated human peripheral blood lymphocytes with emetine-actinomycin D-pretreated cloned human acute lymphatic leukemia cells (CEM). Three assay systems were used to investigate the activity of the macrophage migration inhibitory factor and of the macrophage activation factors for glucose consumption (MAF-G) and for O2- formation. In the culture supernatant of hybridomas and other cells, various combinations of these activities were detected. The results indicate that at least three molecules are concerned in each of these activities.