Postexcision transposition of the transposon Tn10 in Escherichia coli K12.

Molecular & general genetics : MGG ·Vol. 190 ·No. 1 ·1983-00-00 ·Pages 139-42

Bresler SE, Tamm SE, Goryshin IYu, Lanzov VA

Abstract

An experimental analysis of the fate of transposon Tn10 after excision from a proA::Tn10 site localized on the plasmid F' leads to the conclusions: 1. The precise excision is a progressive process. Its probability is estimated per time unit. 2. An excised Tn10 is always integrated into a different genetic locus. 2. An excised Tn10 is always integrated into a different genetic locus. 3. The kinetics of postexcision transposition are sometimes very slow. The excised transposon is inherited in one cell line in spite of cell multiplication. 4. The processes of excision and secondary insertion have no absolute requirement for the recA+ genotype but they are strongly enhanced in recA+ cells. 5. The kinetics of postexcision transposition are strongly dependent on the genetic site from which the transposon was excised. 6. The probability of postexcision transposition is fully determined by the probability of excision and depends on the genotype of the host and many other factors.

MeSH Terms

Bacterial Proteins/genetics DNA Transposable Elements Escherichia coli/genetics F Factor Genes, Bacterial Kinetics Rec A Recombinases

Chemicals

Bacterial Proteins DNA Transposable Elements Rec A Recombinases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bresler S E

Tamm S E

Goryshin IYu

Lanzov V A

References (12)

12 references, click to expand

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Mol Gen Genet. 1981;183(1):139-43 PMID: 7035814
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Inverted repeats of Tn5 are transposable elements.
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2632-5 PMID: 6283538
A derivative of Tn5 with direct terminal repeats can transpose.
J Mol Biol. 1982 Feb 25;155(2):105-20 PMID: 6281443
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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00330336

Published

1983-00-00

Pages

139-42

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00330336

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Postexcision transposition of the transposon Tn10 in Escherichia coli K12.

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