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PMID: 6304023 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Poly(ADP-ribosylation) of a DNA topoisomerase.

The Journal of biological chemistry ·Vol. 258 ·No. 10 ·1983-05-25 ·Pages 6000-3

Ferro AM, Higgins NP, Olivera BM

Abstract

A DNA topoisomerase activity, copurifying with poly(ADP-ribose) synthetase from calf thymus, is greater than 95% inhibited if extensive poly(ADP-ribosylation) is allowed to occur. The inhibited DNA topoisomerase, which has drastically different elution properties on hydroxylapatite, can be reactivated by mild alkaline treatment. These results are consistent with a poly(ADP-ribosylation) of the DNA topoisomerase and covalent attachment of the poly(ADP-ribose) moieties to the topoisomerase by alkali-labile bonds.

MeSH Terms

Animals Cattle Chromatography Chromatography, Gel DNA Topoisomerases, Type I/isolation & purification,metabolism Durapatite Hydrogen-Ion Concentration Hydroxyapatites NAD+ Nucleosidase/metabolism Nucleoside Diphosphate Sugars/metabolism Poly Adenosine Diphosphate Ribose/metabolism Poly(ADP-ribose) Polymerases/isolation & purification,metabolism Thymus Gland/enzymology Topoisomerase I Inhibitors

Chemicals

Hydroxyapatites Nucleoside Diphosphate Sugars Topoisomerase I Inhibitors Poly Adenosine Diphosphate Ribose Durapatite Poly(ADP-ribose) Polymerases NAD+ Nucleosidase DNA Topoisomerases, Type I

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ferro A M

Higgins N P

Olivera B M

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

Published

1983-05-25

Pages

6000-3

Language

English

Region

United States

NLM ID

2985121R

Subset

Grants

NIGMS NIH HHS · GM 25654 · United States

NIGMS NIH HHS · GM 26107 · United States

External Links

PubMed source View journal details

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Poly(ADP-ribosylation) of a DNA topoisomerase.

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