A weak upstream promoter gives rise to long human beta-globin RNA molecules.

Biochemical and biophysical research communications ·Vol. 112 ·No. 3 ·1983-05-16 ·Pages 1041-8

Ley TJ, Nienhuis AW

Abstract

The 5' ends of most normal human beta globin mRNA molecules correspond to a single transcription initiation site, often referred to as the "CAP" site (1-4). Using S1 nuclease mapping and primer extension techniques, we have determined that a minority of beta globin gene transcripts are longer at the 5' ends. These longer molecules comprise about 10% of total beta globin RNA molecules in normal human bone marrow cells and in peripheral blood reticulocytes. The long molecules are transcribed only from the sense strand of DNA and are probably spliced correctly. A DNA segment that includes imperfect "CCAAT" and "TATA" promoter-like sequences begins approximately 150 base pairs (bp) upstream from the normal beta globin gene promoter; this "pseudo-promoter" may function in the initiation of the long globin RNA molecules.

MeSH Terms

Bone Marrow/metabolism Endonucleases Globins/genetics,isolation & purification Humans Operon Peptide Chain Initiation, Translational RNA, Messenger/isolation & purification Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic

Chemicals

RNA, Messenger Globins Endonucleases Single-Strand Specific DNA and RNA Endonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ley T J

Nienhuis A W

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/0006-291x(83)91723-0

Published

1983-05-16

Pages

1041-8

Language

English

Region

United States

NLM ID

0372516

Subset

Databases

GENBANK

J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K02544, M18047, M19067, X00423

External Links

PubMed source DOI: 10.1016/0006-291x(83)91723-0 View journal details

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A weak upstream promoter gives rise to long human beta-globin RNA molecules.

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