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PMID: 6302700 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a viable, noninverting herpes simplex virus 1 genome derived by insertion and deletion of sequences at the junction of components L and S.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 9 ·1983-05-00 ·Pages 2690-4

Poffenberger KL, Tabares E, Roizman B

Abstract

Earlier studies have shown that the DNA of herpes simplex virus 1 consists of two covalently linked components, L and S, each flanked by inverted repeats. The two components can invert, and viral DNA extracted from infected cells or virions consists of equimolar concentrations of four populations differing solely in the orientation of L and S components relative to each other. This paper describes a recombinant virus (1358) generated by an insertion of a chimeric thymidine kinase gene within the reiterated sequences of the S component and deletions that eliminated most of the internal inverted repeats at the junction between the L and S components. A characteristic of 1358 is that the L and S components are frozen in one (prototype) orientation. Inversion of L and S components is therefore not required for the replication of viral DNA.

MeSH Terms
Chromosome Inversion DNA Restriction Enzymes/metabolism DNA, Viral/analysis Electrophoresis, Agar Gel Nucleic Acid Conformation Repetitive Sequences, Nucleic Acid Simplexvirus/genetics
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Poffenberger K L
Tabares E
Roizman B
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.9.2690
Published
1983-05-00
Pages
2690-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393893
Subset
IM
Grants
NIGMS NIH HHS · 5-T32-GM07183-08 · United States
NCI NIH HHS · CA 08494 · United States
NCI NIH HHS · CA 19264 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.9.2690 PMC Full Text View journal details
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