Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain.

FEBS letters ·Vol. 155 ·No. 1 ·1983-05-02 ·Pages 19-24

Ksenzenko M, Konstantinov AA, Khomutov GB, Tikhonov AN, Ruuge EK

Abstract

Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O.2(-) generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O.2(-) generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulates the reaction initiated by antimycin. The results indicate that auto-oxidation of unstable ubisemiquinone formed in centre o of the Q-cycle generates most of the O.2(-) radicals in the cytochrome bc1-site of the mitochondrial respiratory chain.

MeSH Terms

Alkenes/pharmacology Animals Anthraquinones/pharmacology Antimycin A/analogs & derivatives,pharmacology Cattle Dimercaprol/pharmacology Electron Transport Complex III Fatty Acids, Unsaturated Hydroxyquinolines/pharmacology Methacrylates Mitochondria, Heart/drug effects,metabolism Multienzyme Complexes/metabolism NADH, NADPH Oxidoreductases/metabolism Oxygen/metabolism Quinone Reductases/metabolism Strobilurins Superoxides/metabolism Thenoyltrifluoroacetone/pharmacology Thiazoles/pharmacology

Chemicals

Alkenes Anthraquinones Fatty Acids, Unsaturated Hydroxyquinolines Methacrylates Multienzyme Complexes Strobilurins Thiazoles Dimercaprol Superoxides antimycin mucidin nonyl-4-hydroxyquinoline-N-oxide Thenoyltrifluoroacetone funiculosin (anthraquinone) Antimycin A myxothiazol NADH, NADPH Oxidoreductases Quinone Reductases Electron Transport Complex III Oxygen

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Ksenzenko M

Konstantinov A A

Khomutov G B

Tikhonov A N

Ruuge E K

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(83)80200-2

Published

1983-05-02

Pages

19-24

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(83)80200-2 View journal details

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Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain.

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