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PMID: 6300903 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

pH and the recycling of transferrin during receptor-mediated endocytosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 8 ·1983-04-00 ·Pages 2258-62

Dautry-Varsat A, Ciechanover A, Lodish HF

Abstract

At pH 5.4 apotransferrin (iron-free transferrin) binds to cell-surface transferrin receptors to the same extent and with the same affinity as does diferric transferrin at pH 7.0. Apotransferrin is quickly dissociated from its receptor when the pH is raised to 7.0. These and other results strongly support a simple model that explains the cycling of transferrin during a single cycle of receptor-mediated endocytosis. Diferric transferrin binds to cell-surface receptors, and the transferrin-receptor complex is endocytosed. The pH of the endocytic vesicle is lowered to 5.5 or below; this causes dissociation of iron from the transferrin-receptor complex, but apotransferrin remains bound to its receptor. The iron remains within the cell, and the apotransferrin-receptor complex is recycled to the cell surface. Upon encountering the neutral pH of the medium, apotransferrin is dissociated from the cell.

MeSH Terms
Animals Apoproteins/metabolism Asialoglycoprotein Receptor Cell Line Endocytosis Humans Hydrogen-Ion Concentration Iron/metabolism Liver Neoplasms, Experimental Receptor, Insulin/physiology Receptors, Cell Surface/metabolism,physiology Receptors, Transferrin
Chemicals
Apoproteins Asialoglycoprotein Receptor Receptors, Cell Surface Receptors, Transferrin Iron Receptor, Insulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dautry-Varsat A
Ciechanover A
Lodish H F
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.8.2258
Published
1983-04-00
Pages
2258-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393798
Subset
IM
Grants
NIAID NIH HHS · AI-08814 · United States
NIGMS NIH HHS · GM27981 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.8.2258 PMC Full Text View journal details
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