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PMID: 6300781 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Elements in the long terminal repeat of murine retroviruses enhance stable transformation by thymidine kinase gene.

Nucleic acids research ·Vol. 11 ·No. 6 ·1983-03-25 ·Pages 1855-72

Jolly DJ, Esty AC, Subramani S, Friedmann T, Verma IM

Abstract

We have investigated the effects of long terminal repeats (LTRs) of murine retroviruses on the frequency of obtaining stable transfectants by the herpes virus thymidine kinase (TK) gene. The results indicate that addition of LTRs enhances the number of TK+ transformants by 10-20 fold. A 5-12 fold enhancement was also observed when chromosomal DNA from either human or hamster cells was mixed with a plasmid containing LTR sequences and transfected onto LTR- cells. The LTR sequences involved in the enhancement were localized in the region which contains tandem repeats. All other regions of the LTR did not show any enhancement of stable TK+ transfectants. The location or the orientation of the enhancer sequences with respect to the TK gene did not exert any influence on the frequency of transformation. The enhancement effect does not appear to be linked to either increased numbers of chromosomal integrations or elevated levels of transcription of the TK gene.

MeSH Terms
Animals Cell Line Cloning, Molecular Cricetinae Cricetulus DNA Restriction Enzymes DNA, Recombinant/metabolism Female Genes HeLa Cells Humans L Cells/enzymology Mice Ovary Plasmids Repetitive Sequences, Nucleic Acid Retroviridae/genetics Thymidine Kinase/genetics Transfection
Chemicals
DNA, Recombinant Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jolly D J
Esty A C
Subramani S
Friedmann T
Verma I M
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48 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.6.1855
Published
1983-03-25
Pages
1855-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325840
Subset
IM
Grants
NCI NIH HHS · CA-21408 · United States
NCI NIH HHS · CA-24288 · United States
NIGMS NIH HHS · GM-28223 · United States
External Links
PubMed source DOI: 10.1093/nar/11.6.1855 PMC Full Text View journal details
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