On the mechanisms involved in the regulation of the cell-surface receptors for human choriogonadotropin and mouse epidermal growth factor in cultured Leydig tumor cells.
The MA-10 cells are a clonal strain of mouse Leydig tumor cells that have receptors for human choriogonadotropin (hCG) and mouse epidermal growth factor (mEGF). Exposure of the cells to hCG results in a reduction in the number of surface hCG receptors, and little or no change in the number of surface mEGF receptors. On the other hand, exposure of the cells to mEGF results in a reduction in the number of both surface mEGF receptors and surface hCG receptors. In order to study these phenomena, we assumed that the number of surface receptors is determined by the rate at which receptors appear at the surface and by the rate of receptor internalization. When these rates were measured, we found that hCG and mEGF reduce their respective surface receptors by increasing the rate of receptor internalization, and that mEGF reduces the surface hCG receptors by decreasing the rate of appearance of the receptor.
25 references, click to expand
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Evidence for recycling of insulin receptors in isolated rat adipocytes.J Biol Chem. 1981 Nov 25;256(22):11464-70 PMID: 7028734
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Direct linkage of 125I-EGF to cell surface receptors. A useful artifact of chloramine-T treatment.J Biol Chem. 1982 Jan 10;257(1):42-5 PMID: 6273440
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Desensitization to gonadotropins in cultured Leydig tumor cells involves loss of gonadotropin receptors and decreased capacity for steroidogenesis.Proc Natl Acad Sci U S A. 1981 Oct;78(10):6309-13 PMID: 6273862
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Insulin-induced receptor loss in cultured human lymphocytes is due to accelerated receptor degradation.Proc Natl Acad Sci U S A. 1981 Nov;78(11):6917-21 PMID: 7031662
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Evidence for different pathways for the degradation of insulin and insulin receptor in the chick liver cell.J Biol Chem. 1982 Feb 10;257(3):1372-7 PMID: 7035448
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The endocytotic rate constant. A cellular parameter for quantitating receptor-mediated endocytosis.J Biol Chem. 1982 Apr 25;257(8):4222-9 PMID: 6279628
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Kinetics of internalization and recycling of the asialoglycoprotein receptor in a hepatoma cell line.J Biol Chem. 1982 Apr 25;257(8):4230-7 PMID: 6279629
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Insulin-induced down-regulation of insulin receptors in 3T3-L1 adipocytes. Altered rate of receptor inactivation.J Biol Chem. 1982 Apr 25;257(8):4285-91 PMID: 7040381
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Internalization and degradation of receptor-bound human choriogonadotropin in Leydig tumor cells. Fate of the hormone subunits.J Biol Chem. 1982 Nov 25;257(22):13306-11 PMID: 6292185
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Desensitization of steroidogenesis in cultured Leydig tumor cells: role of cholesterol.Proc Natl Acad Sci U S A. 1982 Dec;79(24):7796-800 PMID: 6296852
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Hormonal regulation of testicular luteinizing hormone receptors. Effects on cyclic AMP and testosterone responses in isolated Leydig cells.J Biol Chem. 1977 Dec 25;252(24):9002-9 PMID: 200625
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Gonadotropin binding and stimulation of steroidogenesis in Leydig tumor cells.Proc Natl Acad Sci U S A. 1978 Jan;75(1):99-102 PMID: 203945
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Degradation of receptor-bound human choriogonadotropin by murine Leydig tumor cells.J Biol Chem. 1978 Jul 25;253(14):4892-9 PMID: 209021
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Regulation of epidermal growth factor (EGF) receptor activity during the modulation of protein synthesis.J Cell Physiol. 1979 Apr;99(1):101-6 PMID: 222774
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Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells.J Biol Chem. 1980 Feb 25;255(4):1239-41 PMID: 6243633
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Degradation of the subunits of receptor-bound human choriogonadotropin by Leydig tumor cells.Biochim Biophys Acta. 1980 May 7;629(2):409-17 PMID: 6248122
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Insulin-induced loss of the insulin receptor in IM-9 lymphocytes. A biological process mediated through the insulin receptor.J Biol Chem. 1980 Oct 25;255(20):9860-9 PMID: 7000764
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Regulation of gonadotropin receptors and gonadotropin responses in a clonal strain of Leydig tumor cells by epidermal growth factor.J Biol Chem. 1981 Jan 10;256(1):179-83 PMID: 6256344
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Characterization of several clonal lines of cultured Leydig tumor cells: gonadotropin receptors and steroidogenic responses.Endocrinology. 1981 Jan;108(1):88-95 PMID: 6257492
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On the mechanism of ligand-induced down-regulation of insulin receptor level in the liver cell.J Biol Chem. 1981 Feb 25;256(4):1689-94 PMID: 7007369
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Polypeptide hormone degradation and receptor regulation are coupled to ligand internalization. A direct biochemical and morphologic demonstration.J Biol Chem. 1981 May 10;256(9):4591-7 PMID: 6260804
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