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PMID: 6298095 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mapping the alpha-globin genes in Hb J Mexico carriers.

Human genetics ·Vol. 62 ·No. 2 ·1982-00-00 ·Pages 164-6

Trabuchet G, Morle F, Verdier G, Godet J, Benabadji M, Nigon VM

Abstract

The organization of the alpha-globin genes was studied by restriction endonuclease mapping, in subjects carrying the alpha variant Hb J Mexico. A subject homozygous for Hb J synthesized both Hb J (about 55%) and Hb A and had two alpha loci per chromosome. His restriction site map was found to be identical to that obtained with a normal DNA, except for a mutant Bgl II site which was observed on the Hb J chromosome proximal to the 5' alpha-locus. We have also mapped the DNA of a compound heterozygote for Hb J and alpha-thalassemia, who synthesizes 38% Hb J and we have found a single alpha gene corresponding to a - alpha 3.7 haplotype on one chromosome and two alpha genes, respectively alpha J and alpha A, on the other.

MeSH Terms
Autoradiography Chromosome Mapping DNA Restriction Enzymes/genetics Female Genes, Regulator Globins/genetics Hemoglobin J/genetics Hemoglobins, Abnormal/genetics Heterozygote Homozygote Humans Male Thalassemia/genetics
Chemicals
Hemoglobins, Abnormal Hemoglobin J Globins DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Trabuchet G
Morle F
Verdier G
Godet J
Benabadji M
Nigon V M
References (23)
23 references, click to expand
  1. Genetic and biosynthetic studies of families carrying hemoglobin J alpha Mexico: association of alpha-thalassemia with HbJ.
    Hum Genet. 1978 Jun 9;42(2):189-99 PMID: 669703
  2. Multiple alpha chain loci for human haemoglobins: Hb J-Buda and Hb G-Pest.
    Nature. 1972 Jan 7;235(5332):47-50 PMID: 4550395
  3. The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions.
    Cell. 1980 May;20(1):119-30 PMID: 6446404
  4. Proportion of hemoglobin G Philadelphia (alpha 268 Asn leads to Lys beta 2) in heterozygotes is determined by alpha-globin gene deletions.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6874-8 PMID: 6935689
  5. Mapping the alpha-globin genes in an Algerian HbH patient and his family.
    Blood. 1980 Mar;55(3):511-6 PMID: 7357081
  6. The alpha-globin gene adjacent to the gene for HbQ-alpha 74 Asp replaced by His is deleted, but not that adjacent to the gene for HbG-alpha 30 Glu replaced by Gln; three-fourths of the alpha-globin genes are deleted in HbQ-alpha-thalassemia.
    Blood. 1979 Dec;54(6):1407-16 PMID: 508945
  7. Demonstration of two alpha-globin genes per human haploid genome for normals and Hb J Mexico.
    Eur J Biochem. 1977 Aug 15;78(1):161-5 PMID: 71990
  8. Homozygous cases for hemoglobin J Mexico (alpha54 (E3)Gln replaced by Glu) evidence for a duplicated alpha gene with unequal expression.
    Hemoglobin. 1976-1977;1(1):13-25 PMID: 1052168
  9. Homozygous state for Hb Constant Spring (slow-moving Hb X components).
    Blood. 1974 Feb;43(2):251-9 PMID: 4810076
  10. Two different molecular organizations account for the single alpha-globin gene of the alpha-thalassemia-2 genotype.
    J Clin Invest. 1980 Dec;66(6):1319-25 PMID: 7440717
  11. Hemoglobin Koya Dora: high frequency of a chain termination mutant.
    Am J Hum Genet. 1975 Jan;27(1):81-90 PMID: 1155453
  12. Differentiation of the mRNA transcripts originating from the alpha 1- and alpha 2-globin loci in normals and alpha-thalassemics.
    J Clin Invest. 1981 Aug;68(2):439-46 PMID: 6894931
  13. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  14. alpha-globin gene deletions associated with Hb J Tongariki.
    Br J Haematol. 1982 Jun;51(2):243-9 PMID: 6979350
  15. Characterisation of a new alpha thalassemia 1 defect due to a partial deletion of the alpha globin gene complex.
    Nucleic Acids Res. 1980 Nov 11;8(21):4889-98 PMID: 6255436
  16. Homology and concerted evolution at the alpha 1 and alpha 2 loci of human alpha-globin.
    Nature. 1981 Mar 5;290(5801):26-9 PMID: 7010180
  17. Organization of alpha-globin genes in Hb Hasharon (alpha 47 asp replaced by his) carriers.
    Blood. 1980 Dec;56(6):1145-9 PMID: 6254584
  18. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  19. The duplicated human alpha globin genes lie close together in cellular DNA.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5950-4 PMID: 282616
  20. Organization of alpha-globin genes and mRNA translation in subjects carrying haemoglobin Hasharon (alpha 47 Asp replaced by His) from the Ferrara Region (Northern Italy).
    Eur J Biochem. 1980 Oct;111(1):125-30 PMID: 6254772
  21. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  22. Linkage of alpha G-Philadelphia to alpha-thalassemia in African-Americans .
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4885-9 PMID: 6933536
  23. The duplicated human alpha-globin genes: their relative expression as measured by RNA analysis.
    Cell. 1981 May;24(2):345-51 PMID: 7237552
Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00282308
Published
1982-00-00
Pages
164-6
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00282308 View journal details
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