Expression of a neural type of intermediate filament as a distinguishing feature between oat cell carcinoma and other lung cancers.
Expression of intermediate filaments was studied immunohistologically in oat-cell (6 cases), epidermoid (9 cases), adeno- (7 cases), large-cell anaplastic (3 cases), and bronchioalveolar carcinoma (3 cases), of lung. Affinity-purified antibodies against epithelial (anti-keratin), neural (anti-neurofilament), muscle (anti-desmin) and mesenchymal (anti-vimentin) intermediate filaments were used. In indirect immunofluorescence microscopy of oat-cell carcinomas a positive cytoplasmic fluorescence was seen only with antibodies against neural intermediate filaments, neurofilaments, while no staining of the tumor cells was observed with antibodies against other types of intermediate filaments. On the other hand, all the epidermoid, adeno, anaplastic, and bronchioalveolar carcinomas showed constantly a strong reaction with anti-keratin antibodies in a varying number of cells but no decoration with anti-neurofilament antibodies. The results show that expression of neural intermediate filaments is a major distinguishing feature between oat-cell carcinomas and other lung cancers and suggest that anti-neurofilament antibodies can be used as a diagnostic aid in the surgical pathologic study of pulmonary neoplasms.
27 references, click to expand
-
Alveolar rhabdomyosarcoma. Demonstration of the muscle type of intermediate filament protein, desmin, as a diagnostic aid.Am J Pathol. 1982 Aug;108(2):246-51 PMID: 6765734
-
Variable content of histaminase, L-dopa decarboxylase and calcitonin in small-cell carcinoma of the lung. Biologic and clinical implications.N Engl J Med. 1978 Jul 20;299(3):105-10 PMID: 26872
-
Differential location of different types of intermediate-sized filaments in various tissues of the chicken embryo.Differentiation. 1979;15(1):27-40 PMID: 93557
-
Different intermediate-sized filaments distinguished by immunofluorescence microscopy.Proc Natl Acad Sci U S A. 1978 Oct;75(10):5034-8 PMID: 368806
-
Oat-cell carcinoma of the bronchus.J Pathol Bacteriol. 1959 Oct;78:513-9 PMID: 13795444
-
Immunohistochemical analysis of formaldehyde- and trypsin- or pepsin-treated material.Acta Histochem. 1978;63(2):251-60 PMID: 107708
-
Diversity of cytokeratins. Differentiation specific expression of cytokeratin polypeptides in epithelial cells and tissues.J Mol Biol. 1981 Dec 25;153(4):933-59 PMID: 6177862
-
Immunoperoxidase localization of keratin in human neoplasms: a preliminary survey.Am J Pathol. 1980 Oct;101(1):41-9 PMID: 6160769
-
Filament organization revealed in platinum replicas of freeze-dried cytoskeletons.J Cell Biol. 1980 Jul;86(1):212-34 PMID: 6893451
-
Oat-cell carcinoma of the lung. Its origin and relationship to bronchial carcinoid.Cancer. 1968 Dec;22(6):1163-72 PMID: 5705778
-
Biochemical and immunological identification of cytokeratin proteins present in hepatocytes of mammalian liver tissue.Exp Cell Res. 1981 Feb;131(2):299-318 PMID: 6162655
-
Nasopharyngeal lymphoepithelioma. Histological diagnosis as aided by immunohistochemical demonstration of keratin.Virchows Arch B Cell Pathol Incl Mol Pathol. 1982 Aug;40(2):163-9 PMID: 6182682
-
Antibodies to different intermediate filament proteins. Cell type-specific markers on paraffin-embedded human tissues.Lab Invest. 1981 Nov;45(5):427-34 PMID: 6170833
-
Establishment and characterization of two neoplastic cell lines (U-1285 and U-1568) derived from small cell carcinoma of the lung.Acta Pathol Microbiol Immunol Scand A. 1982 May;90(3):149-58 PMID: 6285665
-
In vivo and in vitro differentiation of neurons and astrocytes in the rat embryo. Immunofluorescence study with neurofilament and glial filament antisera.Dev Biol. 1981 Jul 30;85(2):344-57 PMID: 6790318
-
Intermediate filaments in nervous tissues.J Cell Biol. 1978 Dec;79(3):637-45 PMID: 83322
-
Intermediate filament proteins in the developing chick spinal cord.Dev Biol. 1981 Aug;86(1):40-54 PMID: 7197239
-
Neuronal precursor cells in the chick neural tube express neurofilament proteins.Nature. 1981 Aug 27;292(5826):836-8 PMID: 6791030
-
Intermediate filaments as mechanical integrators of cellular space.Nature. 1980 Jan 17;283(5744):249-256 PMID: 7188712
-
Unifying concept of non-pituitary ACTH-secreting tumors: evidence of common origin of neural-crest tumors, carcinoids, and oat-cell carcinomas.Cancer. 1978 Sep;42(3):1263-9 PMID: 212172
-
High levels of intracellular bombesin characterize human small-cell lung carcinoma.Science. 1981 Dec 11;214(4526):1246-8 PMID: 6272398
-
Expression of intermediate filaments in cultured cells.J Cell Sci. 1981 Aug;50:45-63 PMID: 7033254
-
Preparation of antisera to neurofilament protein from chicken brain and human sciatic nerve.J Comp Neurol. 1977 Dec 15;176(4):645-57 PMID: 72760
-
Cytoskeleton changes in fibroblast adhesion and detachment.J Cell Sci. 1980 Jun;43:379-90 PMID: 6774989
-
Oat cell carcinoma of the bronchus and the carcinoid syndrome.Arch Pathol. 1975 Oct;99(10):513-5 PMID: 172051
-
Expression of intermediate filaments in soft-tissue sarcomas.Int J Cancer. 1982 Nov 15;30(5):541-6 PMID: 6185443
-
Immunohistochemical localisation of keratin in human lung tumours.Virchows Arch A Pathol Anat Histol. 1982;394(3):269-77 PMID: 6176066
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
