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PMID: 6296056 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Relationship between plasmid and chromosomal hemolysin determinants of Escherichia coli.

Journal of bacteriology ·Vol. 153 ·No. 2 ·1983-02-00 ·Pages 846-51

Müller D, Hughes C, Goebel W

Abstract

Plasmid hemolysin (hly) determinants have been shown previously to comprise three cistrons (hlyA, hlyB, hlyC), coding for the synthesis and transport of hemolysin. Using recombinant plasmids as specific probes for these cistrons, we were able to analyze the chromosomal hly determinants of nine Escherichia coli strains which belonged to serotypes O4, O6, O18, and O75 and were isolated from urinary tract infections and fecal flora. The chromosomal hly genes shared extensive sequence homology with the cloned plasmid hly determinant. Nevertheless, small differences were observed, and these were found to lie mainly within cistron A (hlyA), which has been shown to determine the hemolysin protein itself. These fine variations were not specific for the O-serotype.

MeSH Terms

Base Sequence Chromosomes, Bacterial DNA Restriction Enzymes Escherichia coli/classification,genetics Genes Genes, Bacterial Hemolysin Proteins/genetics Nucleic Acid Hybridization Plasmids Serotyping

Chemicals

Hemolysin Proteins DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Müller D

Hughes C

Goebel W

References (23)

23 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.153.2.846-851.1983

Published

1983-02-00

Pages

846-51

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC221704

Subset

External Links

PubMed source DOI: 10.1128/jb.153.2.846-851.1983 PMC Full Text View journal details

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Relationship between plasmid and chromosomal hemolysin determinants of Escherichia coli.

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