Home LiteratureArticle Details
PMID: 6295948 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Restriction endonuclease analysis of DNA from genital isolates of herpes simplex virus type 2.

Infection and immunity ·Vol. 38 ·No. 3 ·1982-12-00 ·Pages 834-42

Maitland NJ, Smith IW, Peutherer JF, Robertson DH, Jones KW

Abstract

High-resolution restriction endonuclease analysis of DNA from multiple herpes simplex virus type 2 isolates from 30 patients at a single clinical center has indicated that herpes simplex virus type 2 DNA sequences are highly conserved. However, the use of six endonucleases, EcoRI, BglII, HindIII, KpnI, BamHI, and SstI, established that each patient was infected by a unique virus. Comparison of virus isolates from the same patient show that the most sequence variation occurred in the terminal and subterminal BamHI fragments. The results suggest that each individual may induce specific variation in the herpes simplex virus type 2 genome and that the results of epidemiological studies based on restriction endonuclease analyses of herpes simplex virus type 2 DNA must be interpreted with care.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Viral Deoxyribonuclease BamHI Female Genes, Viral Herpes Genitalis/microbiology Humans Male Simplexvirus/classification,genetics
Chemicals
DNA, Viral DNA Restriction Enzymes Deoxyribonuclease BamHI
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Maitland N J
Smith I W
Peutherer J F
Robertson D H
Jones K W
References (22)
22 references, click to expand
  1. The specificity of rabbit antisera to Herpesvirus hominis and its dependence on the dose of virus inoculated.
    J Med Microbiol. 1970 May;3(2):267-72 PMID: 4318077
  2. Characterization of genital strains of Herpesvirus hominis.
    Br J Vener Dis. 1973 Aug;49(4):385-90 PMID: 4353618
  3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Physical maps for Herpes simplex virus type 1 DNA for restriction endonucleases Hind III, Hpa-1, and X. bad.
    J Virol. 1976 Oct;20(1):222-33 PMID: 185413
  6. A new method for the isolation of herpes simplex virus type 2 DNA.
    Virology. 1976 Oct 1;74(1):256-8 PMID: 185791
  7. A simple, general procedure for purifying restriction endonucleases.
    Nucleic Acids Res. 1977 Aug;4(8):2561-72 PMID: 909783
  8. Transmission of herpes-simplex virus type 1 in a nursery for the newborn. Identification of viral isolates by D.N.A. "fingerprinting".
    Lancet. 1978 May 6;1(8071):964-6 PMID: 76893
  9. Physical maps for HSV type 2 DNA with five restriction endonucleases.
    J Gen Virol. 1978 May;39(2):259-80 PMID: 206654
  10. Structure of the joint region and the termini of the DNA of herpes simplex virus type 1.
    J Virol. 1978 Aug;27(2):374-87 PMID: 211266
  11. Restriction endonuclease fingerprinting of herpes simplex virus DNA: a novel epidemiological tool applied to a nosocomial outbreak.
    J Infect Dis. 1978 Oct;138(4):488-98 PMID: 213496
  12. A rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology.
    Lancet. 1979 Apr 21;1(8121):849-52 PMID: 86095
  13. The structure and isomerization of herpes simplex virus genomes.
    Cell. 1979 Mar;16(3):481-94 PMID: 222462
  14. Recombination in herpes simplex virus: mapping of mutations and analysis of intertypic recombinants.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:827-40 PMID: 226325
  15. Demonstration of exogenous genital reinfection with herpes simplex virus type 2 by restriction endonuclease fingerprinting of viral DNA.
    J Infect Dis. 1979 Sep;140(3):295-304 PMID: 227967
  16. DNA fingerprinting in investigation of apparent nosocomial acquisition of neonatal herpes simplex.
    J Pediatr. 1980 Jul;97(1):91-3 PMID: 6247474
  17. Structures of two spliced herpes simplex virus type 1 immediate-early mRNA's which map at the junctions of the unique and reiterated regions of the virus DNA S component.
    J Virol. 1981 Jan;37(1):431-44 PMID: 6260993
  18. The structure of herpes simplex virus DNA and its application to molecular epidemiology.
    Ann N Y Acad Sci. 1980;354:279-90 PMID: 6261647
  19. Variations in herpes simplex virus isolated from human ganglia and a study of clonal variation in HSV-1.
    Ann N Y Acad Sci. 1980;354:291-308 PMID: 6261648
  20. The detection of DNA tumour virus-specific RNA sequences in abnormal human cervical biopsies by in situ hybridization.
    J Gen Virol. 1981 Jul;55(Pt 1):123-37 PMID: 6271899
  21. Restriction enzyme analysis of herpesvirus-2 DNA.
    Lancet. 1981 Dec 19-26;2(8260-61):1424 PMID: 6118793
  22. Genital infection with herpes simplex virus type I.
    J Infect. 1982 Jan;4(1):33-5 PMID: 6309976
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.38.3.834-842.1982
Published
1982-12-00
Pages
834-42
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347823
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.38.3.834-842.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2