Proteolytic activation can produce a phosphatidylinositol phosphodiesterase highly sensitive to Ca2+.

The Biochemical journal ·Vol. 206 ·No. 3 ·1982-09-15 ·Pages 675-8

Hirasawa K, Irvine RF, Dawson RM

Abstract

The phosphatidylinositol phosphodiesterase of rat brain shows little activity under conditions likely to pertain in vivo (neutral pH and micromolar Ca(2+) concentrations). A short incubation of a brain supernatant with trypsin, or a longer pre-incubation of the supernatant alone, produce new forms of the enzyme, which are active under such conditions. A possible role of receptor-linked proteinases in initiating phosphatidylinositol catabolism is discussed.

MeSH Terms

Animals Brain/drug effects,enzymology Calcium/pharmacology Enzyme Activation/drug effects In Vitro Techniques Phosphatidylinositol Diacylglycerol-Lyase Phosphoric Diester Hydrolases/metabolism Rats Trypsin/pharmacology

Chemicals

Phosphoric Diester Hydrolases Trypsin Phosphatidylinositol Diacylglycerol-Lyase Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hirasawa K

Irvine R F

Dawson R M

References (23)

23 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2060675

Published

1982-09-15

Pages

675-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1158640

Subset

External Links

PubMed source DOI: 10.1042/bj2060675 PMC Full Text View journal details

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Proteolytic activation can produce a phosphatidylinositol phosphodiesterase highly sensitive to Ca2+.

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