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PMID: 6290987 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of a X. laevis tRNATyr gene in mammalian cells.

Nucleic acids research ·Vol. 10 ·No. 15 ·1982-08-11 ·Pages 4609-26

Laski FA, Alzner-DeWeerd B, RajBhandary UL, Sharp PA

Abstract

Expression of a X. laevis tRNATyr gene has been studied in mammalian cells. This tRNATyr gene has a 13 base intervening sequence adjacent to its anticodon. A fragment containing the tRNATyr gene was cloned into the late region of SV40. Cells infected with a recombinant virus stock vastly overproduce a tRNATyr that is properly spliced, processed and modified. It was also found that the X. laevis tRNATyr is identical or nearly identical to an endogenous tRNATyr of monkey kidney cells. The possibility of using the X. laevis tRNATyr gene to create an amber suppressor for mammalian cells is discussed.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Nucleic Acid Hybridization RNA, Transfer, Amino Acyl/genetics Simian virus 40/genetics Transcription, Genetic Xenopus
Chemicals
DNA, Recombinant RNA, Transfer, Amino Acyl DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Laski F A
Alzner-DeWeerd B
RajBhandary U L
Sharp P A
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.15.4609
Published
1982-08-11
Pages
4609-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321116
Subset
IM
Grants
NIGMS NIH HHS · GM17151 · United States
NCI NIH HHS · P01-CA14051 · United States
NCI NIH HHS · P01CA26717 · United States
External Links
PubMed source DOI: 10.1093/nar/10.15.4609 PMC Full Text View journal details
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