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PMID: 6289307 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Site-directed psoralen crosslinking of DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 15 ·1982-08-00 ·Pages 4594-8

Saffran WA, Goldenberg M, Cantor CR

Abstract

A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing a sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced by nick-translation. Then the psoralen was specifically directed to this site in the dark through a Hg-S linkage prior to irradiation to form a crosslink. The location of interstrand cross-links was examined by electron microscopy of Pst I-cut molecules and found to be at or near the BamHI site.

MeSH Terms
Cross-Linking Reagents DNA DNA Restriction Enzymes Furocoumarins Methods Microscopy, Electron Organomercury Compounds Sulfhydryl Compounds Trioxsalen/analogs & derivatives
Chemicals
Cross-Linking Reagents Furocoumarins Organomercury Compounds Sulfhydryl Compounds 2-pyridyldithioethylmethylamidodiethoxyethane methylaminomethyltrimethylpsoralen DNA DNA Restriction Enzymes Trioxsalen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saffran W A
Goldenberg M
Cantor C R
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.15.4594
Published
1982-08-00
Pages
4594-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346721
Subset
IM
Grants
PHS HHS · GN14825 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.15.4594 PMC Full Text View journal details
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