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PMID: 6288562 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Defective interfering particles of respiratory syncytial virus.

Infection and immunity ·Vol. 37 ·No. 2 ·1982-08-00 ·Pages 439-44

Treuhaft MW, Beem MO

Abstract

A multiplicity-dependent interference was observed in respiratory syncytial virus preparations (Randall strain) grown in HEp-2 cells, and the factor mediating this interference was characterized. Cloned virus did not demonstrate this multiplicity-dependent interference, but its replication was shown to be inhibited by the interfering factor by assays of reduction of infectious yield assay, the interferon factor was found to be particulate, to be inactivated by UV irradiation, and not to interfere with the replication of a heterologous virus, vesicular stomatitis virus. These characteristics are compatible with the physical properties and biological behavior of defective interfering particles. Defective interfering particles were generated by four undiluted passages of cloned virus but were not apparent after eight passages at a multiplicity of infection of 0.1.

MeSH Terms

Cytopathogenic Effect, Viral Respiratory Syncytial Viruses/growth & development Respirovirus Infections/microbiology Stomatitis/microbiology Vesicular stomatitis Indiana virus/growth & development Viral Interference Viral Plaque Assay Virus Diseases/microbiology

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Treuhaft M W

Beem M O

References (13)

13 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.37.2.439-444.1982

Published

1982-08-00

Pages

439-44

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC347553

Subset

Grants

PHS HHS · A 100238 · United States

External Links

PubMed source DOI: 10.1128/iai.37.2.439-444.1982 PMC Full Text View journal details

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