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PMID: 6287846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Production and characterization of a monoclonal antibody to human Type IV collagen.

The American journal of pathology ·Vol. 108 ·No. 3 ·1982-09-00 ·Pages 310-8

Sakai LY, Engvall E, Hollister DW, Burgeson RE

Abstract

We have produced a monoclonal antibody to human basement membrane Type IV collagen. The antibody reacts with the pepsin-resistant, collagenase-sensitive domain of Type IV collagen isolated from placental membranes, but not with human collagens of Types I, II, III, V, 1alpha, 2alpha, and 3alpha. The antibody precipitates biosynthetically labeled human Type IV procollagen, and the precipitate contains both the alpha1 (IV) and alpha2 (IV) chains, suggesting the occurrence of both of these chains within the same triple-helical molecule. When used in indirect immunofluorescence, the antibody gives brilliant staining of basement membranes from a variety of human tissues but does not stain tissues of bovine, canine, rabbit, rat, or mouse origin. It is suggested that this antibody will be of value in research on the structure of human basement membrane collagen, on the distribution of this collagen in various basement membranes, and particularly for the study of basement membranes in normal human development and pathologic processes.

MeSH Terms
Animals Antibodies, Monoclonal/analysis,biosynthesis Basement Membrane/immunology Binding Sites, Antibody/drug effects Collagen/analysis,immunology,pharmacology Electrophoresis, Polyacrylamide Gel Extraembryonic Membranes/immunology Fluorescent Antibody Technique Humans Hybridomas/drug effects,immunology Kidney Cortex/immunology Mice Microbial Collagenase/pharmacology Placenta/immunology Radioimmunoassay
Chemicals
Antibodies, Monoclonal Collagen Microbial Collagenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sakai L Y
Engvall E
Hollister D W
Burgeson R E
References (38)
38 references, click to expand
  1. Identification of a new basement membrane collagen by the aid of a large fragment resistant to bacterial collagenase.
    FEBS Lett. 1979 May 15;101(2):265-8 PMID: 87347
  2. The localization and synthesis of some collagen types in developing mouse embryos.
    Cell. 1979 Apr;16(4):953-65 PMID: 455457
  3. Collagen heterogeneity in human cartilage: identification of several new collagen chains.
    Biochem Biophys Res Commun. 1979 Apr 27;87(4):1124-31 PMID: 465027
  4. Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
    Biochemistry. 1979 Aug 21;18(17):3815-22 PMID: 224919
  5. Immunohistochemical demonstration of basement membrane collagen in normal human skin and in psoriasis.
    J Cutan Pathol. 1979 Apr;6(2):91-5 PMID: 383753
  6. Structural studies on human type IV collagen.
    J Biol Chem. 1979 Oct 10;254(19):9893-900 PMID: 226531
  7. Laminin--a glycoprotein from basement membranes.
    J Biol Chem. 1979 Oct 10;254(19):9933-7 PMID: 114518
  8. Production and characterization of a monoclonal antibody to chicken type I collagen.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3703-7 PMID: 291035
  9. Immunochemical study on basement membrane (type IV) collagens.
    Immunology. 1979 Sep;38(1):109-16 PMID: 92454
  10. Biochemistry and metabolism of basement membranes.
    Int Rev Cytol. 1979;61:167-228 PMID: 389868
  11. High frequencies of antigen-specific hybridomas: dependence on immunization parameters and prediction by spleen cell analysis.
    J Immunol Methods. 1980;32(3):297-304 PMID: 6153209
  12. Monoclonal antibodies to connective tissue macromolecules: type II collagen.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):440-6 PMID: 7356475
  13. Structural basis for apparent heterogeneity of collagens in human basement membranes: type IV procollagen contains two distinct chains.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):745-9 PMID: 6987668
  14. Characterization of antibodies to basement membrane (type IV) collagen in immunohistological studies.
    Immunobiology. 1980 Jan;156(4-5):372-81 PMID: 6989748
  15. Structurally distinct collagen types.
    Annu Rev Biochem. 1980;49:957-1003 PMID: 6157354
  16. 7-S collagen: characterization of an unusual basement membrane structure.
    Eur J Biochem. 1980;108(1):239-50 PMID: 6250829
  17. Collagen polymorphism in the lung. An immunochemical study of pulmonary fibrosis.
    Hum Pathol. 1980 Jul;11(4):353-66 PMID: 6997183
  18. Macromolecular structure of basement membrane collagens.
    FEBS Lett. 1981 Mar 9;125(1):123-8 PMID: 6262125
  19. Minor collagens of chicken hyaline cartilage.
    Biochemistry. 1981 Sep 15;20(19):5443-8 PMID: 7028104
  20. Fibronectin: current concepts of its structure and functions.
    Coll Relat Res. 1981;1(1):95-128 PMID: 7049547
  21. Immunological characterization of the 7-S domain of type IV collagens.
    Coll Relat Res. 1981 Sep;1(5):419-32 PMID: 6809413
  22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  23. Structure and biosynthesis of basement membranes.
    Int Rev Connect Tissue Res. 1973;6:63-104 PMID: 4198817
  24. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  25. Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure.
    Am J Pathol. 1974 Nov;77(2):314-46 PMID: 4614671
  26. Purified antibodies to collagen: an immunofluorescence study of their reaction with tissue collagen.
    Int Arch Allergy Appl Immunol. 1975;48(5):664-79 PMID: 1093976
  27. Liver cirrhosis: immunofluorescence and biochemical studies demonstrate two types of collagen.
    Klin Wochenschr. 1975 Mar 1;53(5):205-8 PMID: 1097805
  28. Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
  29. Immunochemistry of collagens and procollagens.
    Int Rev Connect Tissue Res. 1976;7:61-99 PMID: 57104
  30. Visualization of type I and II collagens in tissue sections by immunohistologic techniques.
    J Immunol. 1976 Jul;117(1):298-303 PMID: 778266
  31. Fetal membrane collagens: identification of two new collagen alpha chains.
    Proc Natl Acad Sci U S A. 1976 Aug;73(8):2579-83 PMID: 1066666
  32. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines.
    Eur J Immunol. 1976 Apr;6(4):292-5 PMID: 825374
  33. Preparation and use in immunohistology of antibodies specific for type I and type III collagen and procollagen.
    J Immunol Methods. 1976;12(1-2):117-24 PMID: 792345
  34. Antibodies to major histocompatibility antigens produced by hybrid cell lines.
    Nature. 1977 Apr 7;266(5602):550-2 PMID: 558524
  35. Antibodies to distinct types of collagens and procollagens and their application in immunohistology.
    J Immunol Methods. 1977;18(1-2):165-82 PMID: 72122
  36. Nature of the collagenous protein in a tumor basement membrane.
    Eur J Biochem. 1978 Mar;84(1):43-52 PMID: 648517
  37. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens.
    Curr Top Microbiol Immunol. 1978;81:115-20 PMID: 567555
  38. Isolation and tissue localization of type AB2 collagen from normal lung parenchyma.
    Am J Pathol. 1979 Feb;94(2):323-31 PMID: 371411
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1982-09-00
Pages
310-8
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1916037
Subset
IM
Grants
NIADDK NIH HHS · AM 19618 · United States
NIADDK NIH HHS · AM 26693 · United States
NICHD NIH HHS · HD 11966 · United States
External Links
PubMed source PMC Full Text View journal details
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