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PMID: 6286949 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amiloride-sensitive sodium channels in rabbit and guinea-pig gall-bladder.

The Journal of physiology ·Vol. 326 ·1982-05-00 ·Pages 21-34

Cremaschi D, Meyer G

Abstract

1. The effects of low amiloride concentrations (less than 2.5 x 10(-5) M) on the apical membrane (Vm) and transepithelial (Vms) potential differences, transepithelial resistance (Rep) and apical/basolateral membrane resistance ratio (Rm/Rs) have been determined in the gall-bladders of the rabbit and guinea-pig. 2. Vm was hyperpolarized, Vms decreased towards negative values, Rm/Rs increased, but Rep remained unchanged. 3. K+ selectivity of the apical membrane was also checked by increasing luminal K+ concentration from 5.9 to 29.4 mM; the corresponding change in the apical electromotive force was much higher in the rabbit than in the guinea-pig. 4. Pre-incubations with Cl--and HCO3--free salines did not modify K+ selectivity in the rabbit, but nearly doubled it in the guinea-pig. 5. Pre-incubations with control solutions containing 10(-5) M-amiloride in the lumen converted the apical membrane of rabbit gall-bladder into a perfectly K+-perm-selective membrane; the same results were obtained in the guinea-pig only on bathing the tissue with Cl-- and HCO3--free salines together with 10(-5) M-amiloride. 6. It is suggested that in the apical membrane of rabbit gall-bladder K+ and Na+ conductances exist, whereas in guinea-pig gall-bladder both K+, Na+ and anion (Cl- and/or HCO3-) conductances are present; in both species conductive Na+ pathways are inhibited by amiloride. .

MeSH Terms
Amiloride/pharmacology Animals Electric Conductivity Epithelium/physiology Gallbladder/drug effects,physiology Guinea Pigs In Vitro Techniques Ion Channels/drug effects,physiology Kinetics Membrane Potentials/drug effects Potassium/pharmacology Pyrazines/pharmacology Rabbits Sodium/metabolism
Chemicals
Ion Channels Pyrazines Amiloride Sodium Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cremaschi D
Meyer G
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33 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1982.sp014174
Published
1982-05-00
Pages
21-34
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1251456
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1982.sp014174 PMC Full Text View journal details
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