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PMID: 6285348 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human papilloma viral DNA replicates as a stable episome in cultured epidermal keratinocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 11 ·1982-06-00 ·Pages 3393-7

LaPorta RF, Taichman LB

Abstract

Human papilloma virus (HPV) is poorly understood because systems for its growth in tissue culture have not been developed. We report here that cultured human epidermal keratinocytes could be infected with HPV from plantar warts and that the viral DNA persisted and replicated as a stable episome. There were 50-200 copies of viral DNA per cell and there was no evidence to indicate integration of viral DNA into the cellular genome. There was also no evidence to suggest that viral DNA underwent productive replication. We conclude that cultured human epidermal keratinocytes may be a model for the study of certain aspects of HPV biology.

MeSH Terms
Cell Division Cells, Cultured DNA, Viral/biosynthesis Epidermal Cells Epidermis/microbiology Humans Papillomaviridae/growth & development Plasmids Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
LaPorta R F
Taichman L B
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.11.3393
Published
1982-06-00
Pages
3393-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346426
Subset
IM
Grants
NIDCR NIH HHS · R01DE04511 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.11.3393 PMC Full Text View journal details
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