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PMID: 6285343 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human transferrin receptor: expression of the receptor is assigned to chromosome 3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 10 ·1982-05-00 ·Pages 3241-5

Enns CA, Suomalainen HA, Gebhardt JE, Schröder J, Sussman HH

Abstract

Human chromosome 3 has been identified as responsible for expression of the transferrin receptor in mouse-human lymphocyte hybrids. The receptor was detected by immunoprecipitation with anti-human receptor antibody of 125I-labeled cells. This method also detected a similar 94,000-dalton protein in mouse cells. A radioimmunoassay developed for the human transferrin receptor measured 10% crossreactivity with the mouse protein. The two proteins were distinguished by NaDodSO4/polyacrylamide gel patterns of partial proteolytic digests of the immunoprecipitated proteins. Mouse-human hybrids were generated by fusing a mouse thymoma (BW5147) cell line to either concanavalin A- or pokeweed mitogen-activated human peripheral blood lymphocytes or a mouse myeloma (NS-1) to uncultured human peripheral blood lymphocytes. Each hybrid was karyotyped with respect to both mouse and human chromosomes. In every case, expression of the human transferrin receptor correlated only with human chromosome 3.

MeSH Terms
Animals Chromosomes, Human, 1-3 Humans Hybrid Cells Mice Molecular Weight Receptors, Cell Surface/analysis,genetics Receptors, Transferrin Transferrin/metabolism
Chemicals
Receptors, Cell Surface Receptors, Transferrin Transferrin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Enns C A
Suomalainen H A
Gebhardt J E
Schröder J
Sussman H H
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.10.3241
Published
1982-05-00
Pages
3241-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346391
Subset
IM
Grants
NCI NIH HHS · CA09151 · United States
NCI NIH HHS · CA13533 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.10.3241 PMC Full Text View journal details
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