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PMID: 6285307 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Primary and secondary structure specificity of the cleavage of 'single-stranded' DNA by endonuclease Hinf I.

Nucleic acids research ·Vol. 10 ·No. 9 ·1982-05-11 ·Pages 2763-73

Hofer B, Ruhe G, Koch A, Köster H

Abstract

The interaction of endonuclease Hinf I with single-stranded fd DNA was examined. The sizes of the cleavage products indicate that the enzyme cuts this substrate at the same sequences as double-stranded DNA (GANTC). To determine whether or not the recognition sites in single-stranded DNA have to be present in double-stranded form in order to be cleaved, DNA fragments containing complementary or non-complementary Hinf I sequences were prepared and treated as substrates. The results suggest that completely base-paired recognition sites are necessary for cleavage. Sequences surrounding the Hinf I pentanucleotides significantly modulate the reaction rates.

MeSH Terms

Base Sequence DNA Restriction Enzymes/metabolism DNA, Single-Stranded Deoxyribonucleases, Type II Site-Specific Molecular Weight Nucleic Acid Conformation Substrate Specificity

Chemicals

DNA, Single-Stranded DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GANTC-specific type II deoxyribonucleases TCGA-specific type II deoxyribonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hofer B

Ruhe G

Koch A

Köster H

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.9.2763

Published

1982-05-11

Pages

2763-73

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320654

Subset

External Links

PubMed source DOI: 10.1093/nar/10.9.2763 PMC Full Text View journal details

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Primary and secondary structure specificity of the cleavage of 'single-stranded' DNA by endonuclease Hinf I.

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