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PMID: 6284959 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Simian virus 40 mutant with transposed T-antigen and VP1 genes.

Journal of virology ·Vol. 41 ·No. 3 ·1982-03-00 ·Pages 1025-37

Lazarowitz SG

Abstract

A simian virus 40 mutant with the T-antigen gene transposed to the late region of the viral genome has been constructed. This transposed molecule directed the synthesis of a full-sized, 92,000-dalton large T antigen in both permissive and nonpermissive cells. This large T antigen functioned in the initiation of viral DNA replication and in the transformation of nonpermissive cells. T-antigen synthesis by this transposed genome had the characteristics of late transcription, thus indicating that functional large T antigen of simian virus 40 is not required for the initiation of late transcription.

MeSH Terms
Animals Antigens, Viral/genetics,physiology Antigens, Viral, Tumor Cell Transformation, Viral DNA Replication Gene Expression Regulation Genes, Viral Mice Mutation Simian virus 40/genetics Viral Proteins/genetics Virus Replication
Chemicals
Antigens, Viral Antigens, Viral, Tumor Viral Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lazarowitz S G
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58 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.41.3.1025-1037.1982
Published
1982-03-00
Pages
1025-37
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256840
Subset
IM
Grants
NCI NIH HHS · 5 P01 CA16519 · United States
External Links
PubMed source DOI: 10.1128/JVI.41.3.1025-1037.1982 PMC Full Text View journal details
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