Nucleotide sequence of the origin of replication in bacteriophage phiX174 RF DNA.

Nature ·Vol. 271 ·No. 5644 ·1978-02-02 ·Pages 417-20

Langeveld SA, van Mansfeld AD, Baas PD, Jansz HS, van Arkel GA, Weisbeek PJ

Abstract

The gene A protein of bacteriophage phiX174 has been used in vitro to convert phiX RFI DNA into the relaxed RFII form by nicking the viral strand. The nucleotide sequence at the 3' end of the nick has been determined as -- T G C T C C C C C A A C T T Goh. This sequence gives the exact position of the origin of phiX RF DNA replication.

MeSH Terms

Base Sequence Coliphages/analysis DNA Replication DNA, Viral Genes Genes, Viral Nucleic Acid Conformation Viral Proteins/metabolism

Chemicals

DNA, Viral Viral Proteins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Langeveld S A

van Mansfeld A D

Baas P D

Jansz H S

van Arkel G A

Weisbeek P J

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/271417a0

Published

1978-02-02

Pages

417-20

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/271417a0 View journal details

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Nucleotide sequence of the origin of replication in bacteriophage phiX174 RF DNA.

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