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PMID: 6281242 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Construction and behavior of strains with mutations in two chemotaxis genes.

Journal of bacteriology ·Vol. 150 ·No. 3 ·1982-06-00 ·Pages 1297-301

DeFranco AL, Koshland DE

Abstract

Double mutants have been constructed by transducing each of the six che genes from the main che gene cluster into the cheC mutant with reversed behavior. The behavioral properties of these double-mutant strains were examined. The results are interpreted in terms of a model based on the cheC gene product being the component of the flagellar basal body that generates tumbling or smooth swimming in response to changes in the level of the response regulator. The properties of the double mutants can then be explained in ways which provide further understanding of the bacterial sensing system.

MeSH Terms
Chemotaxis DNA Transposable Elements Genes, Bacterial Models, Genetic Mutation Salmonella typhimurium/genetics,physiology Transduction, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DeFranco A L
Koshland D E
References (16)
16 references, click to expand
  1. Nonchemotactic mutants of Escherichia coli.
    J Bacteriol. 1967 Jan;93(1):390-8 PMID: 5335897
  2. Genetic analysis of fla and mot cistrons closely linked to H1 in Salmonella abortusequi and its derivatives.
    J Gen Microbiol. 1972 Apr;70(1):59-75 PMID: 5041268
  3. The gradient-sensing mechanism in bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509-12 PMID: 4560688
  4. Nonsense motility mutants in Salmonella typhimurium.
    Genetics. 1973 Feb;73(2):229-45 PMID: 4572290
  5. Quantitation of the sensory response in bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):710-3 PMID: 1091931
  6. Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants.
    J Mol Biol. 1975 Sep 15;97(2):225-35 PMID: 1100857
  7. Chemotactic mechanism of Salmonella typhimurium: preliminary mapping and characterization of mutants.
    J Bacteriol. 1977 Apr;130(1):223-31 PMID: 323229
  8. A response regulator model in a simple sensory system.
    Science. 1977 Jun 3;196(4294):1055-63 PMID: 870969
  9. Normal-to-curly flagellar transitions and their role in bacterial tumbling. Stabilization of an alternative quaternary structure by mechanical force.
    J Mol Biol. 1977 May 5;112(1):1-30 PMID: 328893
  10. Sensory transduction in Escherichia coli: two complementary pathways of information processing that involve methylated proteins.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3312-6 PMID: 333433
  11. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  12. Behavioral genetics in bacteria.
    Annu Rev Genet. 1977;11:397-414 PMID: 339820
  13. Potentiation, desensitization, and inversion of response in bacterial sensing of chemical stimuli.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2820-4 PMID: 351616
  14. Inversion of a behavioral response in bacterial chemotaxis: explanation at the molecular level.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4150-4 PMID: 360210
  15. Biochemistry of sensing and adaptation in a simple bacterial system.
    Annu Rev Biochem. 1981;50:765-82 PMID: 6791579
  16. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1982-06-00
Pages
1297-301
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216354
Subset
IM
Grants
NIADDK NIH HHS · AM 09765 · United States
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