The catalytic mechanism of glutamyl-tRNA synthetase of Escherichia coli. Evidence for a two-step aminoacylation pathway, and study of the reactivity of the intermediate complex.

Adenosine Monophosphate/metabolism Adenosine Triphosphate/metabolism Amino Acyl-tRNA Synthetases/metabolism Catalysis Diphosphates/metabolism Escherichia coli/enzymology Glutamate-tRNA Ligase/metabolism Hydrogen-Ion Concentration Hydroxylamine Hydroxylamines/pharmacology Kinetics Models, Chemical RNA, Transfer, Amino Acyl/metabolism

Diphosphates Hydroxylamines RNA, Transfer, Amino Acyl Hydroxylamine Adenosine Monophosphate Adenosine Triphosphate Amino Acyl-tRNA Synthetases Glutamate-tRNA Ligase