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PMID: 6278491 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic AMP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 12 ·1981-12-00 ·Pages 7825-9

Van Buskirk R, Dowling JE

Abstract

Horizontal cells of the carp retina were separated from other retinal cell types by using enzymatic dissociation and velocity sedimentation at unit gravity. Fractions containing horizontal cells were tested for their ability to accumulate cyclic AMP in the presence of various putative neurotransmitters. Micromolar concentrations of dopamine, when added in the presence of 3-isobutyl-1-methylxanthine, stimulated cyclic AMP accumulation in these isolated cells. The dopamine-dependent accumulation of cyclic AMP in intact isolated horizontal cells was blocked by nanomolar concentrations of dopamine antagonists such as haloperidol, (+)-butaclamol, and fluphenazine. The results indicate that there is a postsynaptic dopamine receptor on carp horizontal cells that is associated with adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1].

MeSH Terms
Adenylyl Cyclases/metabolism Animals Carps Cell Separation/methods Cyclic AMP/metabolism Dopamine/physiology Enzyme Activation Receptors, Dopamine/drug effects,physiology Retina/cytology,metabolism
Chemicals
Receptors, Dopamine Cyclic AMP Adenylyl Cyclases Dopamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Van Buskirk R
Dowling J E
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19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.12.7825
Published
1981-12-00
Pages
7825-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349364
Subset
IM
Grants
NIGMS NIH HHS · 5 T32 GM 07598-04 · United States
NEI NIH HHS · EY-00811 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.12.7825 PMC Full Text View journal details
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