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PMID: 6276549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of sodium-channel density in the natriferic response of the toad urinary bladder to an antidiuretic hormone.

The Journal of membrane biology ·Vol. 64 ·No. 1-2 ·1982-00-00 ·Pages 77-89

Li JH, Palmer LG, Edelman IS, Lindemann B

Abstract

Urinary bladders of Bufo marinus were depolarized, by raising the serosal K concentration, to facilitate voltage-clamping of the apical membrane. Passive Na transport across the apical membrane was then studied with near-instantaneous current-voltage curves obtained before and after eliciting a natriferic response with oxytocin. Fitting with the constant-field equation showed that the natriferic effect is accounted for by an increase in the apical Na permeability. It is accompanied by a small increase in cellular Na activity. Furthermore, fluctuation analysis of the amiloride-induced shot-noise component of the short-circuit current indicated that the permeability increase is not due to increased Na translocation through those Na channels which were already conducting prior to hormonal stimulation. Rather, the natriferic effects is found to be based on an increase in the population of transporting channels. It appears that, in response to the hormone, Na channels are rapidly "recruited" from a pool of electrically silent channels.

MeSH Terms
Animals Biological Transport, Active/drug effects Bufo marinus Electric Conductivity Ion Channels/drug effects,physiology Kinetics Oxytocin/pharmacology Sodium/metabolism Urinary Bladder/drug effects,physiology
Chemicals
Ion Channels Oxytocin Sodium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Li J H
Palmer L G
Edelman I S
Lindemann B
References (32)
32 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01870770
Published
1982-00-00
Pages
77-89
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NIADDK NIH HHS · AM-07219 · United States
NIADDK NIH HHS · AM-13659 · United States
NHLBI NIH HHS · HL-S3115 · United States
External Links
PubMed source DOI: 10.1007/BF01870770 View journal details
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