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PMID: 6272718 Published · ppublish English Journal Article

Photolabelling of membrane proteins with photoactive phospholipids.

The Biochemical journal ·Vol. 193 ·No. 3 ·1981-03-01 ·Pages 757-63

Bisson R, Montecucco C

Abstract

Photoactive probes have been introduced recently to study the hydrophobic sector of integral membrane proteins. A simple procedure to synthesize a new series of highly radioactive aryl azido-phospholipids is presented. They effectively exchange with the boundary lipids and, on illumination, the cross-link to several membrane proteins with high efficiency. The procedure and analysis of labelling of ATPase from sarcoplasmic reticulum is reported as an example. The advantages in using these photoactive phospholipids are discussed together with some information obtained on their use.

MeSH Terms
Adenosine Triphosphatases/metabolism Animals Azides Cattle Electron Transport Complex IV/metabolism Fluorescent Dyes In Vitro Techniques Light Membrane Proteins/metabolism Methods Phospholipids Sarcoplasmic Reticulum/enzymology Sodium-Potassium-Exchanging ATPase/metabolism Spectrophotometry Swine
Chemicals
Azides Fluorescent Dyes Membrane Proteins Phospholipids Electron Transport Complex IV Adenosine Triphosphatases Sodium-Potassium-Exchanging ATPase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bisson R
Montecucco C
References (36)
36 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1930757
Published
1981-03-01
Pages
757-63
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162664
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1930757 PMC Full Text View journal details
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