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PMID: 6271678 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Production of exoenzyme S by clinical isolates of Pseudomonas aeruginosa.

Infection and immunity ·Vol. 34 ·No. 1 ·1981-10-00 ·Pages 147-53

Sokol PA, Iglewski BH, Hager TA, Sadoff JC, Cross AS, McManus A, Farber BF, Iglewski WJ

Abstract

Exoenzyme S differs from toxin A and diphtheria toxin in that it does not adenosine diphosphate (ADP)-ribosylate elongation factor-2, but rather catalyzes the transfer of the ADP-ribose moiety of nicotinamide adenine dinucleotide to a number of different proteins in extracts of eucaryotic cells. Polyoma-transformed BHK-21 cells were isolated which were resistant to diphtheria toxin and toxin A. Extracts from these cells are ADP-ribosylated by exoenzyme S but not toxin A or diphtheria toxin, providing an assay which distinguishes between S and A activities. A total of 124 clinical isolates of P. aeruginosa were analyzed for production of toxin A and exoenzyme S. Exoenzyme S production was detected in 38% of the strains, whereas 80% of the strains produced toxin A.

MeSH Terms
ADP Ribose Transferases Adenosine Diphosphate Ribose/metabolism Animals Bacterial Toxins Biological Assay Cell Line Cell Transformation, Viral Cricetinae Exotoxins/biosynthesis Nucleotidyltransferases/biosynthesis,pharmacology Poly(ADP-ribose) Polymerases Polyomavirus Proteins/metabolism Pseudomonas Infections/microbiology Pseudomonas aeruginosa/enzymology Virulence Factors
Chemicals
Bacterial Toxins Exotoxins Proteins Virulence Factors Adenosine Diphosphate Ribose ADP Ribose Transferases Poly(ADP-ribose) Polymerases exoenzyme S toxA protein, Pseudomonas aeruginosa Nucleotidyltransferases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Sokol P A
Iglewski B H
Hager T A
Sadoff J C
Cross A S
McManus A
Farber B F
Iglewski W J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.34.1.147-153.1981
Published
1981-10-00
Pages
147-53
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350835
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.34.1.147-153.1981 PMC Full Text View journal details
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