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PMID: 6271281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alignment of cloned amiE gene of Pseudomonas aeruginosa with the N-terminal sequence of amidase.

Bioscience reports ·Vol. 1 ·No. 4 ·1981-04-00 ·Pages 299-307

Clarke PH, Drew RE, Turberville C, Brammar WJ, Ambler RP, Auffret AD

Abstract

A restriction enzyme map was constructed for 5.1-kb fragment of Pseudomonas aeruginosa DNA inserted into plasmid pBR322. Restriction enzyme sites were matched to the N-terminal amino acid sequence of amidase to obtain alignment of the amiE gene within the cloned fragment.

MeSH Terms
Amidohydrolases/genetics Amino Acid Sequence Bacteriophage lambda Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Genes Genetic Vectors Plasmids Pseudomonas aeruginosa/genetics
Chemicals
DNA, Recombinant DNA Restriction Enzymes Amidohydrolases amidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Clarke P H
Drew R E
Turberville C
Brammar W J
Ambler R P
Auffret A D
Article Info
Journal
Bioscience reports
Abbr.
Biosci Rep
ISSN
0144-8463
DOI
10.1007/BF01114869
Published
1981-04-00
Pages
299-307
Language
English
Region
England
NLM ID
8102797
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01114869 View journal details
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