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PMID: 6269050 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Quantitation of DNase I sensitivity in Xenopus chromatin containing active and inactive globin, albumin and vitellogenin genes.

Nucleic acids research ·Vol. 9 ·No. 11 ·1981-06-11 ·Pages 2455-74

Felber BK, Gerber-Huber S, Meier C, May FE, Westley B, Weber R, Ryffel GU

Abstract

The disappearance of defined restriction fragments of the beta 1-globin, an albumin and the A1 vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity factors. DNase I digestion of chromatin revealed for the same gene fragments sensitivity factors differing over a much wilder range. This is correlated to the activity of the genes analyzed: the beta 1-globin gene fragment is more sensitive to DNase I in chromatin of erythrocytes compared to hepatocytes whereas the albumin gene fragment is more sensitive to DNase I in chromatin of hepatocytes. The A1 vitellogenin gene has the same DNase I sensitivity in both cell types. Comparing the DNase I sensitivity of the three genes in their inactive state we suggest that different chromatin conformations may exist for inactive genes.

MeSH Terms
Albumins/genetics Animals Base Sequence Chromatin/analysis DNA/genetics DNA Restriction Enzymes Deoxyribonuclease I Deoxyribonucleases/metabolism Endonucleases/metabolism Erythrocytes/metabolism Genes Globins/genetics Lipoproteins/genetics Liver/metabolism Male Nucleic Acid Hybridization Vitellogenins/genetics Xenopus
Chemicals
Albumins Chromatin Lipoproteins Vitellogenins Globins DNA Deoxyribonucleases Endonucleases DNA Restriction Enzymes Deoxyribonuclease I
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Felber B K
Gerber-Huber S
Meier C
May F E
Westley B
Weber R
Ryffel G U
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19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.11.2455
Published
1981-06-11
Pages
2455-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326864
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.11.2455 PMC Full Text View journal details
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