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PMID: 6267426 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1.

Molecular & general genetics : MGG ·Vol. 181 ·No. 4 ·1981-00-00 ·Pages 548-51

Christiansen L, Pedersen S

Abstract

Transducing lambda phages have been isolated that carry segments of the Escherichia coli chromosome in the aspC region, 20.5 min on the E. coli map. One of these phages, lambda aspC2, carries rpsA, the structural gene for the ribosomal protein S1. A three kilobase fragment from this phage, cloned into either the plasmid pACYC184 or the plasmid pBR322, was found to express S1. In cells carrying the rpsA gene on the high copy number plasmid pBR322 the rate of rpsA mRNA synthesis was increased 40-fold, whereas the rate of protein S1 synthesis was doubled, in comparison with these rates in an rpsA haploid.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes/metabolism Escherichia coli/genetics Gene Expression Regulation Genes Plasmids Protein Biosynthesis Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
Ribosomal Proteins ribosomal protein S1 DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Christiansen L
Pedersen S
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21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00428751
Published
1981-00-00
Pages
548-51
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00428751
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