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PMID: 6264484 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2606-10

Moriarty AM, Hoyer BH, Shih JW, Gerin JL, Hamer DH

Abstract

We have constructed a simian virus 40 recombinant carrying a fragment of DNA from hepatitis B virus. Cultured monkey kidney cells infected with this recombinant produce hepatitis B surface antigen. The antigen is excreted into the culture medium as 22-nm particles with the same physical properties, antigenic composition, and constituent polypeptides as those found in the sera of patients with type B hepatitis.

MeSH Terms
Animals Antigens, Surface/genetics Antigens, Viral/genetics Cells, Cultured Chlorocebus aethiops DNA, Recombinant Genes Genes, Viral Genetic Vectors Hepatitis B virus/immunology Immunologic Techniques Simian virus 40/genetics
Chemicals
Antigens, Surface Antigens, Viral DNA, Recombinant
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moriarty A M
Hoyer B H
Shih J W
Gerin J L
Hamer D H
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2606
Published
1981-04-00
Pages
2606-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319398
Subset
IM
Grants
PHS HHS · N01-A1-82572 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.4.2606 PMC Full Text View journal details
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