Elicitation of primary anti-Sendai virus cytotoxic T lymphocytes with purified viral glycoproteins.

The requirement of integration of viral proteins into cell surface membranes of host cells for elicitation of anti-Sendai virus (SV) cytotoxic T lymphocytes (CTL) has been investigated. The purified hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of SV incorporated into phospholipid vesicles was used to analyze this question. Phospholipid vesicles possessing an active HN and F glycoprotein were capable of eliciting both anti-SV CTL and antibodies. However, the incorporation of an inactive F glycoprotein into HN-containing vesicles or its absence from such vesicles resulted in stimulation of only anti-SV antibodies and not anti-SV CTL.