Home LiteratureArticle Details
PMID: 6255200 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of the gene for the polyoma small T antigen in Escherichia coli.

Journal of virology ·Vol. 36 ·No. 1 ·1980-10-00 ·Pages 125-32

Horwich A, Koop AH, Eckhart W

Abstract

A cloned segment of the polyoma virus genome encoding the small T antigen has been fused, in the correct phase for translation, to the 5' end of the beta-galactosidase gene. The hybrid gene, cloned in Escherichia coli, produces a protein resembling the small T antigen.

MeSH Terms
Antigens, Viral/genetics Cloning, Molecular Escherichia coli/genetics Genes, Viral Lysogeny Phenotype Polyomavirus/genetics Protein Biosynthesis
Chemicals
Antigens, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Horwich A
Koop A H
Eckhart W
References (21)
21 references, click to expand
  1. The nature of lactose operator constitive mutations.
    J Mol Biol. 1971 Jul 28;59(2):273-305 PMID: 4935786
  2. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli.
    J Bacteriol. 1972 Jun;110(3):1135-46 PMID: 4555406
  3. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
    Cell. 1974 Dec;3(4):315-25 PMID: 4216403
  4. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  5. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  6. Interaction of bacteriophage T4 RNA and DNA ligases in joining of duplex DNA at base-paired ends.
    J Biol Chem. 1977 Jun 10;252(11):3987-94 PMID: 863910
  7. Cloning of chemically synthesized lactose operators.
    Gene. 1977 Jul;1(5-6):305-21 PMID: 338421
  8. The binding site on SV40 DNA for a T antigen-related protein.
    Cell. 1978 Jan;13(1):165-79 PMID: 202398
  9. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  10. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  11. Characterization of T antigens in polyoma-infected and transformed cells.
    Cell. 1978 Sep;15(1):65-77 PMID: 212199
  12. Tumor antigens induced by nontransforming mutants of polyoma virus.
    Cell. 1978 Oct;15(2):485-96 PMID: 214241
  13. A general method for maximizing the expression of a cloned gene.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):760-4 PMID: 370836
  14. Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens.
    Cell. 1979 Jun;17(2):357-70 PMID: 222480
  15. The nucleotide sequence and genome organization of the polyoma early region: extensive nucleotide and amino acid homology with SV40.
    Cell. 1979 Jul;17(3):715-24 PMID: 225042
  16. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  17. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.
    Nature. 1979 Oct 18;281(5732):544-8 PMID: 386136
  18. Coding potential and regulatory signals of the polyoma virus genome.
    Nature. 1980 Jan 31;283(5746):445-53 PMID: 6243401
  19. Regions of the polyoma genome coding for T antigens.
    Nucleic Acids Res. 1979 Dec 20;7(8):2275-88 PMID: 230465
  20. Synthesis of simian virus 40 t antigen in Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5596-600 PMID: 230483
  21. Plasmids of Escherichia coli as cloning vectors.
    Methods Enzymol. 1979;68:245-67 PMID: 232214
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.36.1.125-132.1980
Published
1980-10-00
Pages
125-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353623
Subset
IM
Grants
NCI NIH HHS · CA 13884 · United States
NCI NIH HHS · CA 14195 · United States
NIGMS NIH HHS · GM 25617 · United States
Databases
GENBANK
M10493
External Links
PubMed source DOI: 10.1128/JVI.36.1.125-132.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2