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PMID: 6255175 Published · ppublish English Journal Article

Analysis of secondary modifications of mouse mammary tumor virus proteins by two-dimensional gel electrophoresis.

Journal of virology ·Vol. 35 ·No. 2 ·1980-08-00 ·Pages 340-8

Nusse R, Janssen H, de Vries L, Michalides R

Abstract

The structural proteins of mouse mammary tumor virus (MMTV) were analyzed by two-dimensional electrophoresis on isoelectric focusing and sodium dodecyl sulfate gels. Many of the viral proteins displayed heterogeneity in charge due to variable contents of carbohydrates (in particular, sialic acid) and phosphate residues. Neuraminidase treatment of the virions influenced the isoelectric pattern of the envelope glycoproteins. The glycoproteins of an MMTV variant which was attenuated by replication in feline kidney cells had different isoelectric points. This suggested that the acquisition of an altered carbohydrate configuration had changed the host range of the virus. The major MMTV structural core protein, p27, consisted of two species, which had identical iodinated tryptic peptide compositions but differed in phosphate contents. Another MMTV phosphoprotein, p21, was separated into four different phosphorylated species. Phosphorylation of p21 could be performed in vitro by the MMTV virion-associated protein kinase. This enzyme also has a high affinity for MMTV p30 as a substrate. Possible functions of this enzyme are discussed.

MeSH Terms
Electrophoresis, Polyacrylamide Gel Glycoproteins/analysis Isoelectric Focusing Mammary Tumor Virus, Mouse/analysis Neuraminidase/pharmacology Phosphoproteins/analysis Viral Proteins/analysis,metabolism
Chemicals
Glycoproteins Phosphoproteins Viral Proteins Neuraminidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nusse R
Janssen H
de Vries L
Michalides R
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45 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.35.2.340-348.1980
Published
1980-08-00
Pages
340-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288818
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.35.2.340-348.1980 PMC Full Text View journal details
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