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PMID: 6254045 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA-transformed murine teratocarcinoma cells: regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 8 ·1980-08-00 ·Pages 4875-9

Linnenbach A, Huebner K, Croce CM

Abstract

Thymidine kinase-deficient (TK-; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21)F9 teratocarcinoma stem cells have been transformed with a recombinant plasmid genome consisting of the pBR322 genome linked to a herpes simplex virus type 1 thymidine kinase gene (HSV-1 tk) and a simian virus 40 (SV40) genome. A clonal line of stem cells was obtained that contains only one copy of plasmid DNA, which is integrated into murine chromosomal DNA through a site on the pBRR322 genome. The HSV-1 tk gene, which is adjacent to the SV40 genome, is expressed in stem cells, whereas SV40 gene expression is not detectable. If differentiation of these stem cells is induced, the differentiated cells express SV40 early gene products. Thus, we have constructed a stem cell which contains a set of genes (SV40), the expression of which is regulated differently in stem and differentiated cells. This cell line could be used to determine the mechanism of suppression of expression of these genes in stem cells.

MeSH Terms
Animals Antigens, Neoplasm/genetics Antigens, Viral/genetics Cell Differentiation Cell Transformation, Viral Gene Expression Regulation Genes, Viral Mice Neoplasms, Experimental Simian virus 40/genetics,immunology Teratoma/genetics,pathology Transfection
Chemicals
Antigens, Neoplasm Antigens, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Linnenbach A
Huebner K
Croce C M
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.8.4875
Published
1980-08-00
Pages
4875-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349951
Subset
IM
Grants
NCI NIH HHS · CA-10815 · United States
NCI NIH HHS · CA-16885 · United States
NCI NIH HHS · CA-20741 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.8.4875 PMC Full Text View journal details
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